{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T06:18:08Z","timestamp":1776925088200,"version":"3.51.2"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust NZ J Obst Gynaeco"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Microsurgical testicular sperm extraction (microTESE) is crucial for treating non\u2010obstructive azoospermia (NOA), offering both \u2018fresh\u2019 and \u2018frozen\u2019 options. This study evaluates the impact of fresh versus frozen microTESE on the progression to intra\u2010cytoplasmic sperm injection (ICSI) cycles, focusing on sperm motility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We conducted a retrospective analysis of microTESE procedures at a major medical centre from 2007 to 2021, excluding cases of obstructive azoospermia and cryptozoospermia. Patients were divided into two groups: fresh microTESE (Group FR) and frozen microTESE (Group FZ). Sperm motility was assessed, and ICSI outcomes were compared between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 128 microTESE procedures on 113 NOA patients, 31 were fresh and 97 were frozen. Sperm was found in 67.7% of fresh cases and 45.3% of frozen cases. In fresh cases, 85.7% had motile sperm for ICSI, whereas in frozen cases, 81.8% had motile sperm initially, but only 52.7% retained motility post\u2010thaw.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings indicate a significant drop in motile sperm availability for ICSI in frozen microTESE cases compared to fresh ones. This suggests a potential advantage of fresh microTESE for certain couples, despite the logistical challenges, highlighting the need for careful patient selection and counselling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ajo.13880","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T20:50:04Z","timestamp":1726606204000},"page":"198-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative motility assessment of sperm retrieved from micro\u2010testicular sperm extraction: A single\u2010centre study comparing fresh and frozen\u2013thawed sperm"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3111-3978","authenticated-orcid":false,"given":"Raneen","family":"Sawaid Kaiyal","sequence":"first","affiliation":[{"name":"Department of Urology, Glickman Urological and Kidney Institute Cleveland Clinic Foundation  Cleveland Ohio 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