{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:41:50Z","timestamp":1787319710789,"version":"build-2736575974"},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust NZ J Obst Gynaeco"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sperm selection tools are used in assisted reproduction to isolate spermatozoa with the least DNA fragmentation (DF) to optimize fertilization, improve pregnancy outcome and reduce genetic abnormalities in the offspring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aims to compare the sperm DF after three different sperm selection tools were performed on split samples from the neat (unprocessed, control) semen sample. (1) Density gradient centrifugation (DGC). (2) Density gradient centrifugation with swim\u2010up (DGC + swim\u2010up). (3) Microfluidic chip (ZyMot). The primary outcome was the DF of spermatozoa after sperm selection tool as measured by Halosperm test. Scientific time taken to perform the test and the cost of consumables were recorded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This was a prospective assessor blinded controlled trial between split semen samples conducted at Care Fertility (Australia) in 2024\/2025 to compare DF after sperm selection. For each sample, a routine semen analysis was performed, and residual sample was split for processing by sperm selection tools.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Thirty participants consented and underwent the trial. Neat (control) semen: mean sperm DF\u2009=\u20099.5% DGC: mean sperm DF\u2009=\u20094.83% AUD34.11 consumables + 60 min scientific time. DGC+Swim\u2010up: mean sperm DF\u2009=\u20091.8% AUD34.73 consumables + 90 min scientific time. Microfluidic Chip mean sperm DF\u2009=\u20090.06% AUD143.46 consumables + 30 min scientific time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Microfluidic chip is highly effective at selecting sperm with the least DF and is faster to perform than DGC +\/\u2212 Swim up.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ajo.70095","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T11:08:42Z","timestamp":1774523322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparing Sperm Selection Tools to Improve\n                    <scp>DNA<\/scp>\n                    Integrity"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6630-4821","authenticated-orcid":false,"given":"Ashleigh","family":"Smith","sequence":"first","affiliation":[{"name":"Consultant (Fertility specialist)  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Badrak","sequence":"additional","affiliation":[{"name":"Consultant (Fertility specialist)  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Pinto","sequence":"additional","affiliation":[{"name":"Care Fertility  Greenslopes Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Alichniewicz","sequence":"additional","affiliation":[{"name":"Consultant (Fertility specialist)  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9320-658X","authenticated-orcid":false,"given":"Clare","family":"Boothroyd","sequence":"additional","affiliation":[{"name":"Care Fertility, Greenslopes Hospital  Greenslopes Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.androl.2022.03.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-7783-9_10"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deu228"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.18502\/ijrm.v19i1.8184"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13874"},{"key":"e_1_2_7_7_1","article-title":"ACCEPT Group Condensed Position Paper on the Management of Recurrent Pregnancy Loss","volume":"65","author":"Suker A.","year":"2025","journal-title":"Australian and New Zealand Journal of Obstetrics and Gynaecology"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep088"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey239"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deac099"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm13040619"}],"container-title":["Australian and New Zealand Journal of Obstetrics and Gynaecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajo.70095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ajo.70095","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajo.70095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:34:09Z","timestamp":1784788449000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/ajo.70095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,26]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/ajo.70095"],"URL":"https:\/\/doi.org\/10.1111\/ajo.70095","archive":["Portico"],"relation":{},"ISSN":["0004-8666","1479-828X"],"issn-type":[{"value":"0004-8666","type":"print"},{"value":"1479-828X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,26]]},"assertion":[{"value":"2025-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70095"}}