{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:41:45Z","timestamp":1787568105848,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100024053","name":"Sydney Local Health District","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust NZ J Obst Gynaeco"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Clinical chorioamnionitis is a major contributor to preterm birth and neonatal morbidity. Diagnosis in women with intact membranes is challenging, particularly in the subclinical phase where maternal signs are subtle and conventional serum markers are limited in predictive value.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To evaluate the diagnostic performance of selected novel amniotic fluid biomarkers (interleukin\u20106, interleukin\u201010, tumour necrosis factor\u2010\u0251, matrix metalloproteinase\u20108), and conventional amniotic and serum biomarkers in predicting delivery for clinical chorioamnionitis within 14\u2009days of amniocentesis in women with suspected subclinical infection and intact membranes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A prospective cohort study was conducted at a single Australian tertiary hospital. Thirty\u2010two women with singleton pregnancies and suspected subclinical chorioamnionitis underwent amniocentesis. Amniotic fluid and serum were analysed for selected biomarkers. The primary outcome was delivery for clinical chorioamnionitis within 14\u2009days of amniocentesis. ROC curves were constructed to assess biomarker performance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Delivery for clinical chorioamnionitis occurred in 11 of 31 evaluable cases (35%). Matrix metalloproteinase\u20108, lactate dehydrogenase, and glucose were the strongest predictors (area under the curve (AUC) of 0.93 (95% CI 0.85\u20131.0), 0.93 (95% CI 0.85\u20131.0), and 0.92 (95% CI 0.8\u20131.0) respectively). Amniotic fluid biomarkers outperformed serum markers (C\u2010reactive protein AUC 0.70 (95% CI 0.49\u20130.91) and white cell count AUC 0.67 (95% CI 0.46\u20130.86)). Novel amniotic fluid biomarkers did not demonstrate superior diagnostic performance over conventional amniotic fluid biomarkers including lactate dehydrogenase and glucose.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Amniotic fluid biomarkers show promise in identifying women with subclinical chorioamnionitis. These findings support further validation of scalable biomarker\u2010based testing to guide early intervention and improve perinatal outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ajo.70168","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:13:24Z","timestamp":1785921204000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of Amniotic Fluid Biomarkers to Predict Preterm Delivery for Clinical Chorioamnionitis in Women With Intact Membranes"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3383-1575","authenticated-orcid":false,"given":"Harold","family":"Baxter","sequence":"first","affiliation":[{"name":"Royal Prince Alfred Hospital, Women and Babies  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5421-4694","authenticated-orcid":false,"given":"Ritu","family":"Mogra","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital, Women and Babies  Sydney New South Wales Australia"},{"name":"Sydney Institute for Women, Children and their Families, Sydney Local Health District  Camperdown New South Wales Australia"},{"name":"Department of Obstetrics and Gynaecology, Faculty of Medicine Western Sydney University  Campbelltown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarika","family":"Gupta","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital, Women and Babies  Sydney New South Wales Australia"},{"name":"Reproduction and Perinatal Centre, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Chan","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital, Women and Babies  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5620-0170","authenticated-orcid":false,"given":"Bradley","family":"de Vries","sequence":"additional","affiliation":[{"name":"Sydney Institute for Women, Children and their Families, Sydney Local Health District  Camperdown New South Wales Australia"},{"name":"Reproduction and Perinatal Centre, Faculty of Medicine and Health University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8867-7061","authenticated-orcid":false,"given":"Rajit","family":"Narayan","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital, Women and Babies  Sydney New South Wales Australia"},{"name":"Sydney Institute for Women, Children and their Families, Sydney Local Health District  Camperdown New South Wales Australia"},{"name":"Department of Obstetrics and Gynaecology, Faculty of Medicine Western Sydney University  Campbelltown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.117680"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.08.040"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2010.96"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jp.2016.59"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000001246"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.11.047"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.104207"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2020.1711725"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1701\u20102163(16)32967\u2010X"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8167276"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.118165"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20100528.2010.02765.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002\u20109378(93)90009\u20108"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2011.07.080"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1701\u20102163(15)30656\u20103"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/01443610600604432"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050802127349"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002\u20109378(95)91358\u20100"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0165\u20100378(97)00020\u2010x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050410001702186"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.735324"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.02.037"}],"container-title":["Australian and New Zealand Journal of Obstetrics and Gynaecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajo.70168","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ajo.70168","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ajo.70168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:13:55Z","timestamp":1787566435000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/ajo.70168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/ajo.70168"],"URL":"https:\/\/doi.org\/10.1111\/ajo.70168","archive":["Portico"],"relation":{},"ISSN":["0004-8666","1479-828X"],"issn-type":[{"value":"0004-8666","type":"print"},{"value":"1479-828X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2025-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70168"}}