{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:19:57Z","timestamp":1792030797220,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T00:00:00Z","timestamp":1348531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ursodeoxycholic acid (<jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content>) is the only known beneficial bile acid with immunomodulatory properties. Ursodeoxycholic acid prevents eosinophilic degranulation and reduces eosinophil counts in primary biliary cirrhosis. It is unknown whether <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> would also modulate eosinophilic inflammation outside the gastrointestinal (<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>) tract, such as eosinophilic airway inflammation seen in asthma. The working mechanism for its immunomodulatory effect is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The immunosuppressive features of <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> were studied <jats:italic>in vivo<\/jats:italic>, in mice, in an ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>)\u2010driven eosinophilic airway inflammation model. To study the mechanism of action of <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content>, we analyzed the effect of <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> on eosinophils, <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells, and dendritic cell (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s). <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> function was studied in greater detail, focussing on migration and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell stimulatory strength <jats:italic>in vivo<\/jats:italic> and interaction with <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells <jats:italic>in vitro<\/jats:italic> as measured by time\u2010lapse image analysis. Finally, we studied the capacity of <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> to influence <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cell interaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ursodeoxycholic acid treatment of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010sensitized mice prior to <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> aerosol challenge significantly reduced eosinophilic airway inflammation compared with control animals. <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s expressed the farnesoid <jats:styled-content style=\"fixed-case\">X<\/jats:styled-content> receptor for <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content>. Ursodeoxycholic acid strongly promoted interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u201012 production and enhanced the migration in <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s. The time of interaction between <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s and T cells was sharply reduced <jats:italic>in vitro<\/jats:italic> by <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> treatment of the <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s resulting in a remarkable <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell cytokine production. Ursodeoxycholic acid\u2010treated <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s have less capacity than saline\u2010treated <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s to induce eosinophilic inflammation <jats:italic>in vivo<\/jats:italic> in <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>alb\/c mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ursodeoxycholic acid has the potency to suppress eosinophilic inflammation outside the <jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> tract. This potential comprises to alter critical function of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s, in essence, the effect of <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> on <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s through the modulation of the <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cell interaction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12019","type":"journal-article","created":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T17:35:40Z","timestamp":1348594540000},"page":"1501-1510","source":"Crossref","is-referenced-by-count":59,"title":["Ursodeoxycholic acid suppresses eosinophilic airway inflammation by inhibiting the function of dendritic cells through the nuclear farnesoid <scp>X<\/scp> receptor"],"prefix":"10.1111","volume":"67","author":[{"given":"M. A. M.","family":"Willart","sequence":"first","affiliation":[{"name":"Laboratory of Immunoregulation and Mucosal Immunology Department of Molecular Biomedical Research Flemish Interuniversity Institute of Biotechnology (VIB) Ghent Belgium"},{"name":"Department of Pulmonary Medicine Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"van Nimwegen","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Grefhorst","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Erasmus MC Rotterdam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Hammad","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation and Mucosal Immunology Department of Molecular Biomedical Research Flemish Interuniversity Institute of Biotechnology (VIB) Ghent Belgium"},{"name":"Department of Pulmonary Medicine Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Moons","sequence":"additional","affiliation":[{"name":"Departments of Gastroenterology and Hepatology Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. C.","family":"Hoogsteden","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Lambrecht","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoregulation and Mucosal Immunology Department of Molecular Biomedical Research Flemish Interuniversity Institute of Biotechnology (VIB) Ghent Belgium"},{"name":"Department of Pulmonary Medicine Ghent University Ghent Belgium"},{"name":"Department of Pulmonary Medicine Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"KleinJan","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20897"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510290618"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.00699.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20630"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(98)00248-1"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840160213"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2006.109934"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.120956"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.22168"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300121"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22668"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22519"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107098200"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306309200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0673"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8107"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28295"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0604325"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.7.3645"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2275"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071087"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20042311"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-7568-6"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80348-2"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.767"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2009.113"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201142018"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610019104"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.8.4090"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.03.044"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.3.1672"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.04.021"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-009-0054-2"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63116-1"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-08-109645"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.12019","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.12019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T03:40:08Z","timestamp":1697427608000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.12019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,25]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1111\/all.12019"],"URL":"https:\/\/doi.org\/10.1111\/all.12019","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,25]]}}}