{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:00:17Z","timestamp":1761418817248},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T00:00:00Z","timestamp":1349308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergic sensitization and diseases have been reported to have a very high and increasing prevalence in elite athletes. Over 80% of allergic athletes are poly\u2010sensitized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims at evaluating the potential diagnostic added value of a microarray technology (Immuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> P<\/jats:styled-content>hadia AB [at present Thermo Fisher Scientific] <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>ppsala, Sweden which detects <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> antibodies to specific or cross\u2010reacting allergen components.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seventy\u2010two poly\u2010sensitized athletes according to skin prick test (<jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>) with different allergic phenotypes (asthma <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>19; rhino\u2010conjunctivitis <jats:italic>n\u00a0<\/jats:italic>=\u00a020; food allergy and\/or oral allergy syndrome <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>13; no clinical symptoms <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>20) and two different control populations (20 poly\u2010sensitized sedentary subjects with respiratory allergy and 20 healthy athletes with negative <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>) were studied for detecting specific <jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content>) both to allergen extracts (<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>mmuno<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">IgE<\/jats:styled-content>) and to allergen components (<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>mmuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content> detected the presence of <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> in 90% of poly\u2010sensitized athletes \u2013 in 96% with symptoms and in 75% without symptoms \u2013 and in 100% of allergic controls. The pattern of positivity towards the 103 components tested differed from subject to subject, even in those with the same sensitization to allergen extract <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content>. Based on the ISAC results, poly\u2010sensitized athletes were classified into the following prototypical patterns, differently represented in the clinical phenotypes studied (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03): (1) One single predominant specific allergen positivity; (2) <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> to two or more non\u2010cross\u2010reacting allergens; (3) <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> to cross\u2010reacting allergens; and (4) <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> to components potentially responsible for severe allergic reactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The Immuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content> represents a useful additional tool for diagnosis and management of poly\u2010sensitized athletes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12029","type":"journal-article","created":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T03:18:15Z","timestamp":1349320695000},"page":"1557-1564","source":"Crossref","is-referenced-by-count":21,"title":["Microarray evaluation of specific <scp>IgE<\/scp> to allergen components in elite athletes"],"prefix":"10.1111","volume":"67","author":[{"given":"M.","family":"Bonini","sequence":"first","affiliation":[{"name":"Institute of Translational Pharmacology Italian National Research Council Rome Italy"},{"name":"Department of Medicine \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Marcomini","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Central Laboratory G. 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