{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:54:11Z","timestamp":1761155651800},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T00:00:00Z","timestamp":1359072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neonatal Bacillus <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>almette\u2013<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>u\u00e9rin (<jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>) vaccination induces vigorous T\u2010helper type 1 (<jats:styled-content style=\"fixed-case\">Th<\/jats:styled-content>1) responses and inhibits allergy\u2010related airway dysfunction, but the exact mechanisms remain unclear. The objective of this study was to address where the <jats:styled-content style=\"fixed-case\">Th<\/jats:styled-content>1 cells induced by neonatal <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> vaccination are generated and stored, and how they are recruited into the inflamed airway for the prevention of allergen\u2010induced airway inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We vaccinated neonatal C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice with <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> in a mouse model of asthma and analyzed the expression and function of <jats:styled-content style=\"fixed-case\">Th<\/jats:styled-content>1 cells <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> vaccination\u2013induced Th1 cells in the local inguinal lymph nodes (<jats:styled-content style=\"fixed-case\">ILN<\/jats:styled-content>) migrated into the lungs upon inhaled ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) challenge in <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010sensitized mice. These <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells in the <jats:styled-content style=\"fixed-case\">ILN<\/jats:styled-content> exhibited potentials of activation, proliferation and cytokine secretion and expressed high levels of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>3. Adoptive transfer of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells from <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>\u2010treated <jats:styled-content style=\"fixed-case\">ILN<\/jats:styled-content> significantly decreased allergic airway responses. In addition, the protective effect of <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> vaccination against allergic airway inflammation was lost upon the excision of the <jats:styled-content style=\"fixed-case\">ILN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data demonstrate that <jats:styled-content style=\"fixed-case\">ILN<\/jats:styled-content> serves as a \u2018weapon\u2019 pool of <jats:styled-content style=\"fixed-case\">Th<\/jats:styled-content>1 cells following <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> vaccination, and these cells are ready for the migration into the inflamed lungs upon the allergen challenge, thereby inhibiting allergen\u2010induced airway disorder.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12099","type":"journal-article","created":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T11:09:04Z","timestamp":1359112144000},"page":"330-338","source":"Crossref","is-referenced-by-count":16,"title":["Distant lymph nodes serve as pools of <scp>Th<\/scp>1 cells induced by neonatal <scp>BCG<\/scp> vaccination for the prevention of asthma in mice"],"prefix":"10.1111","volume":"68","author":[{"given":"G.","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Respiratory Disease People's Hospital of Guangxi Zhuang Autonomous Region Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Pulmonary &amp; 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