{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:15:08Z","timestamp":1791332108407,"version":"4.3.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,9,21]],"date-time":"2013-09-21T00:00:00Z","timestamp":1379721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergy diagnosis by determination of allergen\u2010specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> is complicated by clinically irrelevant <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>, of which the most prominent example is <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> against cross\u2010reactive carbohydrate determinants (<jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content>s) that occur on allergens from plants and insects. Therefore, <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content>s cause numerous false\u2010positive results. Inhibition of <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content>s has been proposed as a remedy, but has not yet found its way into the routine diagnostic laboratory. We sought to provide a simple and affordable procedure to overcome the <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> problem.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples from allergic patients were analysed for allergen\u2010specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>s by different commercial tests (from <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ediwiss, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>hadia and <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>iemens) with and without a semisynthetic <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> blocker with minimized potential for nonspecific interactions that was prepared from purified bromelain glycopeptides and human serum albumin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty two per cent of about 6000 serum samples reacted with <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> reporter proteins. The incidence of anti\u2010<jats:styled-content style=\"fixed-case\">CCD I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> reached 35% in the teenage group. In patients with anti\u2010<jats:styled-content style=\"fixed-case\">CCD I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>, application of the <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> blocker led to a clear reduction in read\u2010out values, often below the threshold level. A much better correlation between laboratory results and anamnesis and skin tests was achieved in many cases. The <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> blocker did not affect test results where <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content>s were not involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Eliminating the effect of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>s directed against <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content>s by inhibition leads to a significant reduction in false\u2010positive <jats:italic>in vitro<\/jats:italic> test results without lowering sensitivity towards relevant sensitizations. Application of the <jats:styled-content style=\"fixed-case\">CCD<\/jats:styled-content> blocker may be worthwhile wherever natural allergen extracts or components are used.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12229","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T21:46:03Z","timestamp":1381527963000},"page":"1269-1277","source":"Crossref","is-referenced-by-count":84,"title":["Inhibition of <scp>I<\/scp>g<scp>E<\/scp> binding to cross\u2010reactive carbohydrate determinants enhances diagnostic selectivity"],"prefix":"10.1111","volume":"68","author":[{"given":"F.","family":"Holzweber","sequence":"first","affiliation":[{"name":"Das Labor Villach Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Svehla","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Natural Resources and Life Sciences Vienna (BOKU) Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Fellner","sequence":"additional","affiliation":[{"name":"Kwizda\/Epignost Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Dalik","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Natural Resources and Life Sciences Vienna (BOKU) Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Stubler","sequence":"additional","affiliation":[{"name":"Labor Dr Rohrer Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Hemmer","sequence":"additional","affiliation":[{"name":"FAZ\u2010 Floridsdorf Allergy Center Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Altmann","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Natural Resources and Life Sciences Vienna (BOKU) 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