{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:38:13Z","timestamp":1791171493687,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T00:00:00Z","timestamp":1402617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003354","name":"Garnett Passe and Rodney Williams Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003354","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Group 2 innate lymphoid cells (<jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2s) were shown to be involved in the initiation and coordination of Th2\u2010type immune responses in allergic disease animal models. Recently, <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2s enrichment was noted in chronic rhinosinusitis (<jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>) patients; however, the role of <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2s in coordinating the Th2 response in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> remains to be elucidated. Here, we characterize the <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2 compartment in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> by investigating the correlations between <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2s, Th2 cells and Th2 cytokines expression in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used flow cytometric analysis of sinonasal mucosal tissues of 29 CRS patients and 5 controls to quantify ILC2s and Th2 cells. Messenger RNA expression levels of IL\u20105, IL\u201013, IL\u201025, IL\u201033, TSLP and GATA3 were determined using <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ILC2s were significantly enriched in nasal polyps (CRSwNP) patients. Multivariate linear regression showed a significant positive association of ILC2 numbers with CRSwNP and allergic CRS and a negative association with the number of previous endoscopic sinus surgeries. Group 2 innate lymphoid cell numbers significantly correlated with Th2 cell frequencies. Messenger RNA expression levels of IL\u20105 and IL\u201013 were increased in CRSwNP compared with controls, while <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of IL\u201025 and GATA3 were significantly reduced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results characterize the complex interactions between <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2s and other Th2 response elements in the context of <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> and suggest that <jats:styled-content style=\"fixed-case\">ILC<\/jats:styled-content>2 enrichment occurs in <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>w<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> and in allergic <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12440","type":"journal-article","created":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T09:21:45Z","timestamp":1402651305000},"page":"1154-1161","source":"Crossref","is-referenced-by-count":154,"title":["Association between Group 2 Innate Lymphoid Cells enrichment, nasal polyps and allergy in Chronic Rhinosinusitis"],"prefix":"10.1111","volume":"69","author":[{"given":"D.","family":"Miljkovic","sequence":"first","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bassiouni","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cooksley","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Hauben","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. \u2010J.","family":"Wormald","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Vreugde","sequence":"additional","affiliation":[{"name":"Department of Surgery Otorhinolaryngology Head and Neck Surgery The Queen Elizabeth Hospital, and the University of Adelaide Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2104"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201212-2227OC"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2617"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2276"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhino12.000"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.22461"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23610"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.02.018"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.109269"},{"key":"e_1_2_9_11_1","first-page":"1278","article-title":"Selective expression of a novel surface molecule by human Th2 cells in vivo","volume":"162","author":"Nagata K","year":"1999","journal-title":"J Immunol Baltim Md 1950"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/130937"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005374"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2131"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130071"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12526"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.07.008"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00713.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0304OC"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2011.07.005"},{"key":"e_1_2_9_21_1","first-page":"2443","article-title":"IL\u201033, a potent inducer of adaptive immunity to intestinal nematodes","volume":"180","author":"Humphreys NE","year":"2008","journal-title":"J Immunol Baltim Md 1950"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.04.081"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21082"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.017"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219482110"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2002"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3430"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20080720"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12374"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.21438"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070406"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.12440","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.12440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:34:01Z","timestamp":1696523641000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.12440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,13]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1111\/all.12440"],"URL":"https:\/\/doi.org\/10.1111\/all.12440","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,13]]}}}