{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:56:53Z","timestamp":1791331013398,"version":"4.3.1"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T00:00:00Z","timestamp":1419206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We established a flow cytometric lymphocyte proliferation test (LPT) for the detection of nickel (Ni) and palladium (Pd) sensitization. Eighty\u2010one consecutive patients with an indication for patch test (PT) were tested by LPT with Ni (NiSO<jats:sub>4<\/jats:sub>) and Pd (Na<jats:sub>2<\/jats:sub>PdCl<jats:sub>4<\/jats:sub> and PdCl<jats:sub>2<\/jats:sub>) salts. The imprecision of the LPT was low (coefficient of variation 7.2%). Using PT as a diagnostic reference, the sensitivity and specificity of LPT were 74.4% and 80% for NiSO<jats:sub>4<\/jats:sub>, 74.4% and 78.3% for Na<jats:sub>2<\/jats:sub>PdCl<jats:sub>4<\/jats:sub>, and 57.2% and 85.4% for PdCl<jats:sub>2<\/jats:sub>, respectively. For both Ni and Pd, the likelihood ratio for a positive PT markedly increased with increasing LPT value. With medical history as a reference, the sensitivity and specificity were 40.6% and 82.1% for <jats:styled-content style=\"fixed-case\">LPT<\/jats:styled-content> and 59.4% and 89.7% for PT, respectively. Combination of <jats:styled-content style=\"fixed-case\">LPT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> resulted in a higher specificity of 95%, albeit lower sensitivity of 34.4%. In conclusion, flow cytometric <jats:styled-content style=\"fixed-case\">LPT<\/jats:styled-content> represents a reliable and useful method for the detection of Ni and Pd sensitization. <jats:styled-content style=\"fixed-case\">LPT<\/jats:styled-content> values correlate with <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> results and, when used in combination with PT, increase test specificity.<\/jats:p>","DOI":"10.1111\/all.12553","type":"journal-article","created":{"date-parts":[[2014,12,2]],"date-time":"2014-12-02T08:35:34Z","timestamp":1417509334000},"page":"323-327","source":"Crossref","is-referenced-by-count":18,"title":["Detection of nickel and palladium contact hypersensitivity by a flow cytometric lymphocyte proliferation test"],"prefix":"10.1111","volume":"70","author":[{"given":"I.","family":"Spoerri","sequence":"first","affiliation":[{"name":"Research Group of Dermatology Department of Biomedicine University Hospital Basel University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Scherer","sequence":"additional","affiliation":[{"name":"Allergy Unit Department of Dermatology University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Michel","sequence":"additional","affiliation":[{"name":"Allergy Unit Department of Dermatology University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Link","sequence":"additional","affiliation":[{"name":"Medical Immunology, Laboratory Medicine University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Bircher","sequence":"additional","affiliation":[{"name":"Allergy Unit Department of Dermatology University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. A. F. M.","family":"Heijnen","sequence":"additional","affiliation":[{"name":"Medical Immunology, Laboratory Medicine University Hospital Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2012.02834.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2011.01878.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03862.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1990.tb08276.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10623.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.01963.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb00690.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01045.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90236-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.02.012"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-012-0324-x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(99)00167-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20166"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02070.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2012.02061.x"},{"issue":"3","key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S1081-1206(10)60811-3","article-title":"Contact dermatitis: a practice parameter","volume":"97","author":"American Academy of Allergy, Asthma and Immunology","year":"2006","journal-title":"Ann Allergy Asthma Immunol"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.12553","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.12553","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T01:05:18Z","timestamp":1696467918000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.12553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,22]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/all.12553"],"URL":"https:\/\/doi.org\/10.1111\/all.12553","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,22]]}}}