{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:38:15Z","timestamp":1791499095982,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011947","name":"Abbott Nutrition","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011947","id-type":"DOI","asserted-by":"crossref"}]},{"name":"McMaster University Department of Medicine"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The prebiotic nature of human milk oligosaccharides (\n                      <jats:styled-content style=\"fixed-case\">HMO<\/jats:styled-content>\n                      s) and increasing evidence of direct immunomodulatory effects of these sugars suggest that they may have some therapeutic potential in allergy. Here, we assess the effect of two\n                      <jats:styled-content style=\"fixed-case\">HMO<\/jats:styled-content>\n                      s, 2\u2019\u2010fucosyllactose and 6\u2019\u2010sialyllactose, on symptomatology and immune responses in an ovalbumin\u2010sensitized mouse model of food allergy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The effects of oral treatment with 2\u2032\u2010fucosyllactose and 6\u2032\u2010sialyllactose on anaphylactic symptoms induced by oral ovalbumin (\n                      <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\n                      ) challenge in sensitized mice were investigated. Mast cell functions in response to oral\n                      <jats:styled-content style=\"fixed-case\">HMO<\/jats:styled-content>\n                      treatment were also measured in the passive cutaneous anaphylaxis model, and direct effects on IgE\u2010mediated degranulation of mast cells were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Daily oral treatment with 2\u2032\u2010fucosyllactose or 6\u2032\u2010sialyllactose attenuated food allergy symptoms including diarrhea and hypothermia. Treatment with\n                      <jats:styled-content style=\"fixed-case\">HMO<\/jats:styled-content>\n                      s also suppressed antigen\u2010induced increases in mouse mast cell protease\u20101 in serum and mast cell numbers in the intestine. These effects were associated with increases in the\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      4\n                      <jats:sup>+<\/jats:sup>\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      25\n                      <jats:sup>+<\/jats:sup>\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201010\n                      <jats:sup>+<\/jats:sup>\n                      cell populations in the Peyer's patches and mesenteric lymph nodes, while 6\u2032\u2010sialyllactose also induced increased\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201010 and decreased\n                      <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                      production in antigen\u2010stimulated splenocytes. Both 2\u2032\u2010fucosyllactose and 6\u2032\u2010sialyllactose reduced the passive cutaneous anaphylaxis response, but only 6\u2032\u2010sialyllactose directly inhibited mast cell degranulation in\u00a0vitro, at high concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our results suggest that 2\u2032\u2010fucosyllactose and 6\u2032\u2010sialyllactose reduce the symptoms of food allergy through induction of\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201010\n                      <jats:sup>+<\/jats:sup>\n                      T regulatory cells and indirect stabilization of mast cells. Thus, human milk oligosaccharides may have therapeutic potential in allergic disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.12650","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T03:55:51Z","timestamp":1431489351000},"page":"1091-1102","source":"Crossref","is-referenced-by-count":175,"title":["Attenuation of food allergy symptoms following treatment with human milk oligosaccharides in a mouse model"],"prefix":"10.1111","volume":"70","author":[{"given":"L.","family":"Castillo\u2010Courtade","sequence":"first","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine McMaster University Hamilton ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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