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This study aimed to explore the involvement of TSLP<\/jats:styled-content>, IL<\/jats:styled-content>\u201025, IL<\/jats:styled-content>\u201033, and their receptors in type 2 T\u2010helper (Th) responses in chronic rhinosinusitis with nasal polyps (CRS<\/jats:styled-content>wNP<\/jats:styled-content>s) and their cross\u2010regulation in human nasal epithelial cells (HNEC<\/jats:styled-content>s).<\/jats:p><\/jats:sec>Methods<\/jats:title>Immunohistochemistry, quantitative RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>, ELISA<\/jats:styled-content>, Bio\u2010Plex assay, and flow cytometry were used to detect the expression of TSLP<\/jats:styled-content>\/common \u03b3\u2010like TSLP<\/jats:styled-content> receptor (TSLPR<\/jats:styled-content>)\/IL<\/jats:styled-content>\u20107 receptor \u03b1 (IL<\/jats:styled-content>\u20107R\u03b1), IL<\/jats:styled-content>\u201025\/IL<\/jats:styled-content>\u201017B receptor (IL<\/jats:styled-content>\u201017RB<\/jats:styled-content>), and IL<\/jats:styled-content>\u201033\/membrane\u2010bound ST<\/jats:styled-content>2 (ST<\/jats:styled-content>2L)\/soluble ST<\/jats:styled-content>2 (sST<\/jats:styled-content>2) in sinonasal mucosa and HNEC<\/jats:styled-content>s. HNEC<\/jats:styled-content>s cultured at an air\u2013liquid interface were used to explore the expression in regulation of these cytokine systems.<\/jats:p><\/jats:sec>Results<\/jats:title>Compared with controls and noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, the expression of TSLP<\/jats:styled-content>\/TSLPR<\/jats:styled-content>\/IL<\/jats:styled-content>\u20107R\u03b1 and ST<\/jats:styled-content>2L\/sST<\/jats:styled-content>2 was significantly increased in eosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, predominantly in epithelial cells. In contrast, the expression of IL<\/jats:styled-content>\u201033 and IL<\/jats:styled-content>\u201025\/IL<\/jats:styled-content>\u201017RB<\/jats:styled-content> was enhanced in epithelial cells in both eosinophilic and noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content> compared to controls. The expression of TSLP<\/jats:styled-content>, TSLPR<\/jats:styled-content>, and ST<\/jats:styled-content>2L was positively correlated with symptom and computer tomography scan scores in eosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content> and with Th2 cytokine expression in sinonasal mucosa. The expression of ST<\/jats:styled-content>2L was correlated with TSLP<\/jats:styled-content> and its receptor expression. TSLP<\/jats:styled-content> could induce ST<\/jats:styled-content>2L expression that promoted IL<\/jats:styled-content>\u201033\u2010induced TSLP<\/jats:styled-content> expression in HNEC<\/jats:styled-content>s. In addition, TSLP<\/jats:styled-content>\/TSLPR<\/jats:styled-content>\/IL<\/jats:styled-content>\u20107R\u03b1 and ST<\/jats:styled-content>2L could be induced by Th2 cytokines, while IL<\/jats:styled-content>\u201025\/IL<\/jats:styled-content>\u201017RB<\/jats:styled-content> and IL<\/jats:styled-content>\u201033 could be upregulated by Th1\/Th17 cytokines, in HNEC<\/jats:styled-content>s.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The positive feedback loop between TSLP<\/jats:styled-content>, IL<\/jats:styled-content>\u201033 and their receptors, and Th2 cytokines may facilitate Th2\u2010skewed inflammation in eosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12667","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T14:49:27Z","timestamp":1434725367000},"page":"1169-1180","source":"Crossref","is-referenced-by-count":135,"title":["Interaction of thymic stromal lymphopoietin, IL<\/scp>\u201033, and their receptors in epithelial cells in eosinophilic chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"70","author":[{"given":"B.","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.\u2010P.","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 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