{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:29:58Z","timestamp":1790461798697,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Helsinki University Research Funds"},{"name":"Finnish Allergy Research Foundation"},{"name":"ThermoFisher Scientific"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Component\u2010resolved diagnostics offers a modern tool in peanut allergy, but studies applying consistently double\u2010blind placebo\u2010controlled challenges are lacking. We aimed to optimize diagnostics for moderate\u2010to\u2010severe peanut allergy in a birch\u2010endemic region and to create an oral\u2010peanut challenge with its allergen activity characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed double\u2010blind placebo\u2010controlled peanut challenges for a referred sample of 6\u2010 to 18\u2010year\u2010olds with peanut sensitization or a high suspicion of peanut allergy, including anaphylaxis. We measured specific IgE (<jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content>) to Ara h 1, 2, 3, 6, 8, and 9. Testing of allergen activity of the challenge products was by IgE microarray inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 102 patients, 69 were challenge positive: 25 (36%) had severe, 36 (52%) moderate, and 8 (12%) mild symptoms; 38 (37%) received adrenalin. <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>gE to Ara h 6 <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content> 0.98 (95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 0.96\u20131.00) was the best marker of moderate\u2010to\u2010severe allergy. When <jats:styled-content style=\"fixed-case\">sIgE<\/jats:styled-content> to Ara h 2 and Ara h 6 was measured together, all (100%) severe reactions at low doses were successfully diagnosable. <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>gE to Ara h 8 had no diagnostic value, <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content> 0.42 (95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 0.30\u20130.52). Both nonroasted and roasted peanut inhibited 100% of IgE binding to Ara h 1, 2, 3, and 6. Nonroasted peanut inhibited 87% of IgE binding to Ara h 8, roasted inhibited 30%. The products lacked Ara h 9 activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Co\u2010sensitization to Ara h 2 and Ara h 6 was associated with severe reactions distinguishing severe allergy from mild symptoms. <jats:styled-content style=\"fixed-case\">SI<\/jats:styled-content>gE to Ara h 8 added no diagnostic value. Component\u2010resolved diagnostics reduce the need for oral challenges in peanut allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.12671","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T18:04:37Z","timestamp":1434737077000},"page":"1239-1245","source":"Crossref","is-referenced-by-count":149,"title":["Ara h 2 and Ara 6 are the best predictors of severe peanut allergy: a double\u2010blind placebo\u2010controlled study"],"prefix":"10.1111","volume":"70","author":[{"given":"A. K.","family":"Kukkonen","sequence":"first","affiliation":[{"name":"The Skin and Allergy Hospital University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S.","family":"Pelkonen","sequence":"additional","affiliation":[{"name":"The Skin and Allergy Hospital University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"M\u00e4kinen\u2010Kiljunen","sequence":"additional","affiliation":[{"name":"The Skin and Allergy Hospital University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Voutilainen","sequence":"additional","affiliation":[{"name":"The Skin and Allergy Hospital University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"M\u00e4kel\u00e4","sequence":"additional","affiliation":[{"name":"The Skin and Allergy Hospital University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02176.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.11.020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu2070652"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02561.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02460.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.09.010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8355-x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12530"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12299"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.09.014"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.05.019"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2012.10.004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.10.008"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02555.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.06.008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02312.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03543-1"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.008"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12527"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.12.012"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2012.11.002"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000321108"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.01.056"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12075"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12424"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12047"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00666.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-6-17"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.08.011"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.031"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-013-0369-5"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053465"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.12671","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.12671","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T23:55:24Z","timestamp":1696377324000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.12671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":32,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/all.12671"],"URL":"https:\/\/doi.org\/10.1111\/all.12671","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7]]}}}