{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:35:50Z","timestamp":1771655750643,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC, CIRG12Nov033"],"award-info":[{"award-number":["NMRC, CIRG12Nov033"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170897"],"award-info":[{"award-number":["81170897"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81441028"],"award-info":[{"award-number":["81441028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670909"],"award-info":[{"award-number":["81670909"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Aberrant epithelial remodeling with the ectopic expression of p63 (basal cell markers) is an important pathologic phenomenon seen in chronically inflamed airway epithelium such as in nasal polyps (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Biopsies were obtained from 55 <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients and 18 healthy controls (inferior turbinate). Among <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients, 15 were treated with oral and nasal steroids, so that two sets of <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> biopsies were taken before and after the treatments. p63, Ki67, type <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content> \u03b2\u2010tubulin, and cell cycle markers were investigated in these specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The number of p63<jats:sup>+<\/jats:sup> cells is significantly higher in both hyperplastic (1.53\u2010fold, <jats:italic>P<\/jats:italic> &lt; 0.0001) and squamous metaplastic (2.02\u2010fold, <jats:italic>P<\/jats:italic> &lt; 0.0001) epithelium from <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>s than from healthy controls. There are three types of proliferative basal cells (p63<jats:sup>+<\/jats:sup>\/Ki67<jats:sup>+<\/jats:sup>) which are in different phases of the cell cycle, such as G1 phase (type I cells), S to G2 phase (type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cells), and mitosis (type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> cells). Of importance, some type I cells may arrest after proliferation although they may still be p63<jats:sup>+<\/jats:sup>\/Ki67<jats:sup>+<\/jats:sup>. In healthy epithelium, the ratio of the type I and <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cells is almost 50:50. However, less type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cells are found in hyperplastic epithelium (34.85%, <jats:italic>P<\/jats:italic> = 0.012) and in squamous metaplastic epithelium (30.77%, <jats:italic>P<\/jats:italic> = 0.02) together with the presence of type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> cells (3.45%, <jats:italic>P<\/jats:italic> = 0.01). These findings were not changed after steroid treatments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>An increase of poorly proliferated basal cells forming multiple layers, which may stain for basal cell markers but does not form a proper epidermal barrier, is an important histopathologic phenomenon in aberrant remodeled epithelium of <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13074","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T19:52:13Z","timestamp":1479325933000},"page":"975-984","source":"Crossref","is-referenced-by-count":34,"title":["Increase of poorly proliferated p63<sup>+<\/sup>\/Ki67<sup>+<\/sup> basal cells forming multiple layers in the aberrant remodeled epithelium in nasal polyps"],"prefix":"10.1111","volume":"72","author":[{"given":"L.","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Otolaryngology The Second Hospital of Shandong University Shandong University Jinan China"},{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. W.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. N.","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. T. N.","family":"Dung","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The Second Hospital of Shandong University Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,23]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"European Position Paper on Rhinosinusitis and Nasal Polyps 2012","volume":"23","author":"Fokkens WJ","year":"2012","journal-title":"Rhinol Suppl"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12211"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.12.011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4888"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2002.01343.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70262-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2976"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.22950"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200607-1062OC"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2005.01.012"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12070-012-0485-x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-009-1179-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2012.02842.x"},{"key":"e_1_2_9_15_1","first-page":"464","article-title":"Analysis of Ki\u201067 immunoreactivity in recurring and nonrecurring nasal polyps","volume":"39","author":"Kosem M","year":"2010","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH628>3.0.CO;2-J"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200004)190:5<537::AID-PATH577>3.0.CO;2-W"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04619"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.07.038"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5293.1672"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05360.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.4.501"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.12.1501"},{"key":"e_1_2_9_24_1","first-page":"227","article-title":"Increased epithelial cell proliferation in nasal polyps","volume":"101","author":"Hsu MC","year":"2002","journal-title":"J Formos Med Assoc"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323000703500208"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02402.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.13076"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.46"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.031"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890160072013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.01938.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12480"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2013.07.008"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2233"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.13074","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.13074","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.13074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T15:31:55Z","timestamp":1696347115000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.13074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,23]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/all.13074"],"URL":"https:\/\/doi.org\/10.1111\/all.13074","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,23]]}}}