{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:27:11Z","timestamp":1780493231855,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T00:00:00Z","timestamp":1491264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81325006"],"award-info":[{"award-number":["81325006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570899"],"award-info":[{"award-number":["81570899"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630024"],"award-info":[{"award-number":["81630024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81200733"],"award-info":[{"award-number":["81200733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400448"],"award-info":[{"award-number":["81400448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400449"],"award-info":[{"award-number":["81400449"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Autophagy is a lysosomal degradation pathway that is essential for cell survival, differentiation, and homeostasis. This study aimed to investigate the contribution of autophagy to the pathogenesis of CRS<\/jats:styled-content> with nasal polyps (CRS<\/jats:styled-content>wNP<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>The expression of autophagic proteins [microtubule\u2010associated protein 1 light chain 3B (LC<\/jats:styled-content>3B)\u2010II<\/jats:styled-content>, autophagy\u2010related proteins (Atg), and Beclin 1], substrate proteins (p62 and ubiquitinated proteins), and apoptotic signaling molecules [cysteine\u2010aspartic protease\u20103 and cysteine\u2010aspartic protease\u20108, and poly\u2010ADP<\/jats:styled-content>\u2010ribose polymerase] in the sinonasal mucosa and nasal epithelial cells (NEC<\/jats:styled-content>s) was detected by immunohistochemistry and Western blotting. Autophagic vacuoles were observed with transmission electron microscopy. BEAS<\/jats:styled-content>\u20102B cells and NEC<\/jats:styled-content>s were treated with rapamycin, bafilomycin A1, or various cytokines. In some experiments, cultured NEC<\/jats:styled-content>s were transfected with small interfering RNA<\/jats:styled-content> targeting p62 (sip62) or Atg5 (siAtg5). Cultured cells were analyzed with Western blotting and flow cytometry.<\/jats:p><\/jats:sec>Results<\/jats:title>Although autophagic protein expression and autophagic vacuole formation were increased in both eosinophilic and noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, particularly in NEC<\/jats:styled-content>s, there was also an up\u2010regulation of substrate proteins and apoptotic signaling molecules. IFN<\/jats:styled-content>\u2010\u03b3, but not IL<\/jats:styled-content>\u20104, IL<\/jats:styled-content>\u201013, or IL<\/jats:styled-content>\u201017A, simultaneously enhanced LC<\/jats:styled-content>3B\u2010II<\/jats:styled-content> and p62 levels as well as cell apoptosis in BEAS<\/jats:styled-content>\u20102B cells and\/or normal NEC<\/jats:styled-content>s. Bafilomycin A1 up\u2010regulated the levels of LC<\/jats:styled-content>3B\u2010II<\/jats:styled-content> and p62 in polyp NEC<\/jats:styled-content>s and IFN<\/jats:styled-content>\u2010\u03b3\u2010treated normal NEC<\/jats:styled-content>s. IFN<\/jats:styled-content>\u2010\u03b3\u2010induced apoptosis of normal NEC<\/jats:styled-content>s was exaggerated by bafilomycin A1 and siAtg5. Sip62 suppressed apoptosis of polyp NEC<\/jats:styled-content>s and IFN<\/jats:styled-content>\u2010\u03b3\u2010treated NEC<\/jats:styled-content>s. IFN<\/jats:styled-content>\u2010\u03b3 protein levels were increased in both eosinophilic and noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>IFN<\/jats:styled-content>\u2010\u03b3 induces activated but insufficient autophagy and thus contributes to a degree to p62\u2010dependent apoptosis of NEC<\/jats:styled-content>s in CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13153","type":"journal-article","created":{"date-parts":[[2017,3,4]],"date-time":"2017-03-04T14:42:54Z","timestamp":1488638574000},"page":"1384-1397","source":"Crossref","is-referenced-by-count":33,"title":["Interferon\u2010\u03b3\u2010induced insufficient autophagy contributes to p62\u2010dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"72","author":[{"given":"B\u2010F.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u2010P.","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z\u2010C.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z\u2010Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z\u2010Z.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y\u2010K.","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X\u2010B.","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4168-6702","authenticated-orcid":false,"given":"Z.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.10.010"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.10.009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12296"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12542"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.018"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.09.023"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003316"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e32835b1150"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.09.033"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21456"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin14.232"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2016.30.4282"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.028"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.3678"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1100356"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13540"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370213489446"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2021"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.20297"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1017190"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.7.1576"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.03.015"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.017"},{"key":"e_1_2_7_26_1","first-page":"1","article-title":"European position paper on rhinosinusitis and nasal polyps 2012","volume":"23","author":"Fokkens WJ","year":"2012","journal-title":"Rhinol Suppl"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12667"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001603"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.05.024"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902148.2015.1033569"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/1947-2714.128482"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2014.02.001"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201402-0234OC"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12758"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.77"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.04.052"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2011.08.022"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3532"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1056967"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3205"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12168"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.21403"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.09.031"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201214"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.07.036"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100815200"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.13153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.13153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T01:54:34Z","timestamp":1695002074000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.13153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,4]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/all.13153"],"URL":"https:\/\/doi.org\/10.1111\/all.13153","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,4]]}}}