{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:13:58Z","timestamp":1790745238085,"version":"4.1.0"},"reference-count":8,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T00:00:00Z","timestamp":1499817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Thermo Fisher"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>There is no standardized method for assessing serum total mast cell tryptase (MCT<\/jats:styled-content>) in anaphylaxis. The consensus equation (peak MCT<\/jats:styled-content> should be>1.2\u00d7 baseline tryptase+2 mg\/L) has been proposed to interpret acute MCT<\/jats:styled-content> in mast cell activation syndrome (MCAS<\/jats:styled-content>). To validate consensus equation in a perioperative setting analyses of cases of suspected perioperative anaphylaxis during general anaesthesia (GA<\/jats:styled-content>) were performed. Anaphylaxis was defined as per World Allergy Organisation (WAO<\/jats:styled-content>) criteria. Timed serial MCT<\/jats:styled-content> measurements were mapped against the consensus equation and receiver operating characteristic (ROC<\/jats:styled-content>) curves produced. A total of 82 patients (60 females, mean age 56.5 years\u00b1SD<\/jats:styled-content>17.2) underwent investigation. Sixty (73%) patients fulfilled WAO<\/jats:styled-content> criteria for anaphylaxis, and 22 patients did not. Aetiology included 59% IgE\u2010mediated anaphylaxis, 2% non\u2010IgE\u2010mediated anaphylaxis, 12% anaphylaxis of unknown cause and 27% deemed non\u2010anaphylaxis. IgE\u2010mediated anaphylaxis included the following: NMBA<\/jats:styled-content> (35%), antibiotics (46%), chlorhexidine (8%), patent blue dye (8%) and others (8%). An acute MCT<\/jats:styled-content> with a comparable baseline was available in 71 of 82 (87%) patients (60 anaphylaxis and 11 controls). The median interquartile range (IQR<\/jats:styled-content>) time from reaction to peak MCT<\/jats:styled-content> was 1.34 (0.82\u20102.51) hours. Analyses confirmed that a rise in acute MCT<\/jats:styled-content> greater than that defined by the equation had a sensitivity, specificity, positive predictive value (PPV<\/jats:styled-content>) and negative (N) PV<\/jats:styled-content> of 78%, 91%, 98% and 44%, respectively. The magnitude of increase in acute MCT<\/jats:styled-content> above the threshold predicted by consensus equation was higher in the anaphylaxis group compared to controls (P<\/jats:italic>=.0001). This equation has a high specificity, PPV<\/jats:styled-content> with a moderate NPV<\/jats:styled-content> and sensitivity in perioperative anaphylaxis.<\/jats:p>","DOI":"10.1111\/all.13226","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T19:38:49Z","timestamp":1497382729000},"page":"2031-2034","source":"Crossref","is-referenced-by-count":101,"title":["Validation of international consensus equation for acute serum total tryptase in mast cell activation: A perioperative perspective"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0233-3904","authenticated-orcid":false,"given":"R. L.","family":"Baretto","sequence":"first","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"},{"name":"Department of Immunology Kettering General Hospital NHS Foundation Trust Kettering UK"},{"name":"Department of Biochemistry & Immunology University Hospitals of Coventry and Warwickshire Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Beck","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Heslegrave","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Melchior","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ekbote","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Huissoon","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"},{"name":"Institute of Immunology & Immunotherapy University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. T.","family":"Krishna","sequence":"additional","affiliation":[{"name":"Department of Allergy & Immunology Heart of England NHS Foundation Trust Birmingham UK"},{"name":"Institute of Immunology & Immunotherapy University of Birmingham Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328760"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03404.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01347.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12468"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e318211496c"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12424"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200309000-00007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12785"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.13226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.13226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T19:22:47Z","timestamp":1696015367000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.13226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,12]]},"references-count":8,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/all.13226"],"URL":"https:\/\/doi.org\/10.1111\/all.13226","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,12]]}}}