{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T16:27:52Z","timestamp":1786292872681,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T00:00:00Z","timestamp":1502755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006360","name":"Bundesministerium f\u00fcr Wirtschaft und Energie","doi-asserted-by":"publisher","award":["KF 2784702SB4"],"award-info":[{"award-number":["KF 2784702SB4"]}],"id":[{"id":"10.13039\/501100006360","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl\u2010\u03b1\u2010(1,3)\u2010galactose (\u03b1\u2010Gal). It is known that \u03b1\u2010Gal is immunogenic in humans and causes glycan\u2010specific IgG and also IgE responses with clinical relevance. \u03b1\u2010Gal is part of the IgE\u2010reactive monoclonal therapeutic antibody cetuximab (CTX) and is associated with delayed anaphylaxis to red meat. In this study, different \u03b1\u2010Gal\u2010containing analytes are examined in singleplex and multiplex assays to resolve individual sensitization patterns with IgE against \u03b1\u2010Gal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three serum groups, \u03b1\u2010Gal\u2010associated meat allergy (<jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>) patients, idiopathic anaphylaxis (<jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>) patients with suspected MA, and non\u2010meat\u2010allergic healthy control individuals (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>), were analyzed via singleplex allergy diagnostics and a newly established immunoblot diagnostic system. The new dot blot detection system resolved individual IgE sensitization profiles for \u03b1\u2010Gal\u2010containing analytes CTX, bovine thyroglobulin (Bos d TG), and human serum albumin <jats:bold>(<\/jats:bold><jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>)\u2010conjugated \u03b1\u2010Gal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Singleplex allergy diagnostics using the \u03b1\u2010Gal analytes CTX and Bos d TG confirms the history of MA patients in 91% and 88% of the cases, respectively. A novel dot blot\u2010based assay system for the detection of IgE against \u03b1\u2010Gal reveals individual IgE sensitization profiles for \u03b1\u2010Gal\u2010containing analytes. An \u03b1\u2010Gal\u2010associated IgE cross\u2010reactivity profile (IgE against CTX, Bos d TG, and <jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>\u2010\u03b1\u2010Gal) was identified, which is associated with MA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Detection of individual sensitization patterns with different \u03b1\u2010Gal\u2010containing analytes provides the basis for an individual allergy diagnosis for \u03b1\u2010Gal\u2010sensitized patients. Higher amounts of \u03b1\u2010Gal in pork and beef innards compared to muscle meat as indicated by a higher staining intensity are a plausible explanation for the difference in allergic symptom severity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13238","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T07:22:40Z","timestamp":1499066560000},"page":"93-105","source":"Crossref","is-referenced-by-count":47,"title":["Meat allergy associated with galactosyl\u2010\u03b1\u2010(1,3)\u2010galactose (\u03b1\u2010Gal)\u2014Closing diagnostic gaps by anti\u2010\u03b1\u2010Gal IgE immune profiling"],"prefix":"10.1111","volume":"73","author":[{"given":"U.","family":"Jappe","sequence":"first","affiliation":[{"name":"Division of Clinical and Molecular Allergology Priority Research Area Asthma &amp; Allergy Research Center Borstel Airway Research Center North (ARCN) Member of the German Center for Lung Research (DZL) Borstel Germany"},{"name":"Interdisciplinary Allergy Division Department of Internal Medicine University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Minge","sequence":"additional","affiliation":[{"name":"Division of Clinical and Molecular Allergology Priority Research Area Asthma &amp; Allergy Research Center Borstel Airway Research Center North (ARCN) Member of the German Center for Lung Research (DZL) Borstel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Kreft","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology Martin\u2010Luther\u2010University Halle\u2010Wittenberg Halle (Saale) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Klinikum Augsburg Augsburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Przybilla","sequence":"additional","affiliation":[{"name":"Department of Dermatology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Dermatology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Seidel","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Biedermann","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of T\u00fcbingen T\u00fcbingen Germany"},{"name":"Department of Dermatology and Allergology Technical University of Munich Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Anem\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kromminga","sequence":"additional","affiliation":[{"name":"IPM Biotech Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ru\u00ebff","sequence":"additional","affiliation":[{"name":"Department of Dermatology Ludwig\u2010Maximilians University Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Merk","sequence":"additional","affiliation":[{"name":"Department of Dermatology RWTH Aachen University Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Dermatology Klinikum Darmstadt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Treudler","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology University of Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3449-1245","authenticated-orcid":false,"given":"M.","family":"Worm","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Allergy Center Charit\u00e9 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Waldmann","sequence":"additional","affiliation":[{"name":"Dermatology &amp; 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