{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T18:44:56Z","timestamp":1770230696434,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:00:00Z","timestamp":1502323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004963","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["312147"],"award-info":[{"award-number":["312147"]}],"id":[{"id":"10.13039\/501100004963","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergy can be diagnosed using basophil tests. Several methods measuring basophil activation are available. This study aimed at comparing basophil activation test (<jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content>), histamine release assay (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>), and passive sensitization histamine release assay (passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>) in the diagnosis of peanut allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> HR<\/jats:styled-content>, and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> were performed on 11 peanut\u2010allergic and 14 nonallergic subjects. Blood was incubated with peanut extract or anti\u2010IgE and tests were performed as follows: <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content>\u2014<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 upregulation was assessed by flow cytometry; <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>\u2014released histamine was quantified by a glass fiber\u2010based fluorometric method; passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>\u2014IgE\u2010stripped donor basophils were incubated with participants' serum and histamine release was quantified as <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>sens, a measure of basophil allergen sensitivity, was significantly higher for <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> (80.1\u00b117.4) compared to <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> (23.4\u00b110.31) and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> (11.1\u00b12.0). <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> HR<\/jats:styled-content>, and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> had a clinical sensitivity of 100%, 100%, and 82% and specificity of 100%, 100%, and 100%, respectively, when excluding inconclusive results. <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> identified 11 of 11 allergic patients, <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 10, and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 9. Likewise, <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> recognized 12 of 14 nonallergic subjects, <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 10, and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 13. However, the tests' diagnostic performances were not statistically different. Interestingly, nonreleasers in <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> but not in <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> had lower basophil count compared to releasers (249 vs 630 counts\/min).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> displayed a significantly higher <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>sens compared to <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> and passive <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>. The basophil tests' diagnostic performances were not significantly different. Still, <jats:styled-content style=\"fixed-case\">BAT<\/jats:styled-content> could diagnose subjects with low basophil number in contrast to <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13243","type":"journal-article","created":{"date-parts":[[2017,7,7]],"date-time":"2017-07-07T13:55:48Z","timestamp":1499435748000},"page":"137-144","source":"Crossref","is-referenced-by-count":52,"title":["A comparative study on basophil activation test, histamine release assay, and passive sensitization histamine release assay in the diagnosis of peanut allergy"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6433-3961","authenticated-orcid":false,"given":"L. 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N.","family":"Clare Mills","sequence":"additional","affiliation":[{"name":"Division of Infection, Immunity and Respiratory School of Biological Sciences Manchester Institute of Biotechnology Manchester Academic Health Sciences Centre The University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"van Ree","sequence":"additional","affiliation":[{"name":"Departments of Experimental Immunology and of Otorhinolaryngology Academic Medical Center University of Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. K.","family":"Poulsen","sequence":"additional","affiliation":[{"name":"Allergy Clinic Copenhagen University Hospital Gentofte Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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