{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:39:59Z","timestamp":1791499199223,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Grass pollen\u2010related seasonal allergic rhinoconjunctivitis (<jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>g) is clinically heterogeneous in severity, comorbidities, and response to treatment. The component\u2010resolved diagnostics disclosed also a high heterogeneity at molecular level. Our study aimed at analyzing the characteristics of the IgE sensitization to <jats:italic>Phleum pratense<\/jats:italic> molecules and investigating the diagnostic relevance of such molecules in childhood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined 1120 children (age 4\u201018\u00a0years) with <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>g. Standardized questionnaires on atopy were acquired through informatics platform (Allergy<jats:styled-content style=\"fixed-case\">CARD<\/jats:styled-content>\u2122). Skin prick tests were performed with pollen extracts. Serum IgE to airborne allergens and eight <jats:italic>P.\u00a0pratense<\/jats:italic> molecules (<jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 1, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 2, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 4, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 5b, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 6, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 7, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 11, <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>hl p 12) were tested by ImmunoCAP FEIA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The analysis of IgE responses against eight <jats:italic>P.\u00a0pratense<\/jats:italic> molecules showed 87 profiles. According to the number of molecules recognized by IgE, the more complex profiles were characterized by higher serum total IgE, higher grass\u2010specific serum IgE, and higher number and degree of sensitization to pollens. The most frequent IgE sensitization profile was the monomolecular Phl p 1. Sensitization to Phl p 7 was a reliable biomarker of asthma, whereas Phl p 12 of oral allergy syndrome. Sensitization to Phl p 7 was associated with a higher severity of <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>g, and complex profiles were associated with longer disease duration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In a large pediatric population, the complexity of IgE sensitization profiles against <jats:italic>P.\u00a0pratense<\/jats:italic> molecules is related to high atopic features although useless for predicting the clinical severity. The detection of serum IgE to Phl p 1, Phl p 7, and Phl p 12 can be used as clinical biomarkers of <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>g and comorbidities. Further studies in different areas are required to test the impact of different IgE molecular profiles on <jats:styled-content style=\"fixed-case\">AIT<\/jats:styled-content> response.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13338","type":"journal-article","created":{"date-parts":[[2017,10,21]],"date-time":"2017-10-21T02:34:41Z","timestamp":1508553281000},"page":"673-682","source":"Crossref","is-referenced-by-count":53,"title":["Diagnostic relevance of IgE sensitization profiles to eight recombinant <i>Phleum pratense<\/i> molecules"],"prefix":"10.1111","volume":"73","author":[{"given":"F.","family":"Cipriani","sequence":"first","affiliation":[{"name":"Department of Pediatric Pneumology and Immunology Charit\u00e9 Medical University Berlin Germany"},{"name":"Pediatric Unit Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Mastrorilli","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology and Immunology Charit\u00e9 Medical University Berlin Germany"},{"name":"Pediatric Clinic of Medicine and Surgery University of Parma Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Tripodi","sequence":"additional","affiliation":[{"name":"Pediatric Department and Pediatric Allergology Unit Sandro Pertini Hospital Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Ricci","sequence":"additional","affiliation":[{"name":"Pediatric Unit Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Perna","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology and Immunology Charit\u00e9 Medical University Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Panetta","sequence":"additional","affiliation":[{"name":"Biostatistics L'Altra Statistica srl, Consultancy &amp; Training Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8277-1700","authenticated-orcid":false,"given":"R.","family":"Asero","sequence":"additional","affiliation":[{"name":"Allergology Service San Carlo Clinic Paderno Dugnano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Dondi","sequence":"additional","affiliation":[{"name":"Pediatric Emergency Unit S. 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