{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:31:05Z","timestamp":1789389065864,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Therapeutic tolerance restoration has been proven to modify food allergy in patients and animal models and although sublingual immunotherapy (<jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>) has showed promise, combined therapy may be necessary to achieve a strong and long\u2010term tolerance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>In this work, we combined<jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>with systemic administration of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 associated with an anti\u2010<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 monoclonal antibody (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102\/anti\u2010<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102Ab complex or<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102C) to reverse the IgE\u2010mediated experimental allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Balb\/c mice were sensitized with cholera toxin and milk proteins and orally challenged with allergen to elicit hypersensitivity reactions. Then, allergic mice were treated with a sublingual administration of very low amounts of milk proteins combined with intraperitoneal injection of low doses of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102C. The animals were next re\u2010exposed to allergens and mucosal as well as systemic immunological parameters were assessed<jats:italic>in\u00a0vivo<\/jats:italic>and<jats:italic>in\u00a0vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The treatment reduced serum specific IgE,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105 secretion by spleen cells and increased<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 and<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 in the lamina propria of buccal and duodenal mucosa. We found an augmented frequency of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010\u2010secreting<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>regulatory T cells (Treg) in the submaxilar lymph nodes and buccal lamina propria. Tregs were sorted, characterized and adoptively transferred to na\u00efve mice, which were subsequently sensitized. No allergy was experienced in these mice and we encouragingly discovered a faster and more efficient tolerance induction with the combined therapy compared with<jats:styled-content style=\"fixed-case\">SLIT<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The combination of two therapeutic strategies rendered Treg\u2010mediated tolerance more efficient compared to individual treatments and reversed the established IgE\u2010mediated food allergy. This approach highlights the ability of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102C to expand Tregs, and it may represent a promising disease\u2010modifying therapy for managing food allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13402","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T17:24:05Z","timestamp":1515605045000},"page":"885-895","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Systemic<scp>IL<\/scp>\u20102\/anti\u2010<scp>IL<\/scp>\u20102Ab complex combined with sublingual immunotherapy suppresses experimental food allergy in mice through induction of mucosal regulatory T cells"],"prefix":"10.1111","volume":"73","author":[{"given":"P. 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