{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:21:09Z","timestamp":1780593669157,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T00:00:00Z","timestamp":1517356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["25461583"],"award-info":[{"award-number":["25461583"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["16K10018"],"award-info":[{"award-number":["16K10018"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["15ek0410019h0101"],"award-info":[{"award-number":["15ek0410019h0101"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cutaneous exposure to food antigen through impaired skin barrier has been shown to induce epicutaneous sensitization, thereby causing IgE\u2010mediated food allergies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We examined whether skin barrier impairment following epicutaneous sensitization exacerbates food allergies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were epicutaneously sensitized by repeated application of ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) to <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>903\u2010pretreated ear skin for 48\u00a0hours weekly and then intragastrically challenged with <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>. After the first oral challenge, the skin barrier was disrupted with topical application of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>903 or by tape\u2010stripping. Mice were monitored for changes in body temperature and the occurrence of diarrhea after undergoing the second oral challenge. Serum levels of mouse mast cell protease\u20101 (mmcp1) and <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010specific IgE, IgG1, IgG2a antibodies and <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010specific IgA levels in intestinal lavage fluid were measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Tissue accumulation of eosinophils was determined histologically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Epicutaneously sensitized mice developed anaphylaxis after intragastric challenge, as evidenced by diarrhea, decreased body temperature, and increased serum mmcp1 levels. Skin barrier disruption by <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>903 treatment or tape\u2010stripping exacerbated allergic reactions induced by oral challenge. <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>903 treatment increased serum baseline and postchallenge mmcp1 levels. Topical pretreatment with dexamethasone alleviated allergic reactions that were exacerbated by <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>903 treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Even after eliminating exposure to the antigen, inflammation from skin barrier disruption can exacerbate the severity of food allergy symptoms. Serum baseline mmcp1 levels might be an effective marker for predicting the severity of antigen\u2010induced allergic symptoms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13404","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T17:24:12Z","timestamp":1515605052000},"page":"1313-1321","source":"Crossref","is-referenced-by-count":43,"title":["Skin inflammation exacerbates food allergy symptoms in epicutaneously sensitized mice"],"prefix":"10.1111","volume":"73","author":[{"given":"A.","family":"Kawasaki","sequence":"first","affiliation":[{"name":"Department of Pediatrics Faculty of Medical Sciences University of Fukui Fukui Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Faculty of Medical Sciences University of Fukui Fukui 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