{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:31:27Z","timestamp":1790652687142,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Biologie Servier, France"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n There is in vitro evidence that T cells from allergic patients react to benzylpenicillin\u2010human serum albumin (\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n ) bioconjugates. Our group has recently shown the existence of na\u00efve\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells recognizing\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n in healthy donors. However,\n BP<\/jats:styled-content>\n \u2010haptenated peptides from\n HSA<\/jats:styled-content>\n participating in the immunization of allergic patients have never been identified. The purpose of the present study is to identify immunodominant\n BP<\/jats:styled-content>\n \u2010haptenated peptides from\n HSA<\/jats:styled-content>\n involved in immunization of patients to\n BP<\/jats:styled-content>\n and to refine the frequency calculation of na\u00efve\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells recognizing\n BP<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Co\u2010cultures were established with\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells from non\u2010allergic donors and mature autologous dendritic cells (\n DC<\/jats:styled-content>\n s) loaded with\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n or\n BP<\/jats:styled-content>\n \u2010haptenated peptides from\n HSA<\/jats:styled-content>\n . The\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T\u2010cell response specific for\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n or for individual\n BP<\/jats:styled-content>\n \u2010haptenated peptides was measured using an interferon\u2010\u03b3 (\n IFN<\/jats:styled-content>\n \u2010\u03b3)\n ELIS<\/jats:styled-content>\n pot assay. The frequency of\n BP<\/jats:styled-content>\n \u2010specific\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells was then calculated using the Poisson distribution.\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n and\n BP<\/jats:styled-content>\n \u2010haptenated peptides recognition by allergic patients was evaluated on peripheral blood mononuclear cells (\n PBMC<\/jats:styled-content>\n s) using a lymphocyte transformation test (\n LTT<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Results showed that\n BP<\/jats:styled-content>\n \u2010\n HSA<\/jats:styled-content>\n and\n BP<\/jats:styled-content>\n \u2010haptenated peptides were recognized by na\u00efve T cells from 15\/16 and 13\/14 tested healthy donors, respectively. Most donors responded to 3 peptides with\n BP<\/jats:styled-content>\n covalently bound on lysines 159, 212, and 525. Two of these benzylpenicilloylated peptides (lysines 159 and 525) were also found to induce\n PBMC<\/jats:styled-content>\n s proliferation in patients with allergic reaction to penicillins.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n This study identifies and characterizes for the first time the\n BP<\/jats:styled-content>\n \u2010haptenated peptides from\n HSA<\/jats:styled-content>\n involved in the immunization of patients to penicillins.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/all.13418","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T11:55:20Z","timestamp":1516622120000},"page":"1662-1672","source":"Crossref","is-referenced-by-count":38,"title":["Identification of T\u2010cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy"],"prefix":"10.1111","volume":"73","author":[{"given":"M. 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