{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:34:34Z","timestamp":1777624474517,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T00:00:00Z","timestamp":1518480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003406","name":"Tekes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003406","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Allergen\u2010specific immunotherapy (SIT) effectively alleviates type I allergic diseases characterized by T helper (Th)2\u2010type immunity. Our recent studies have shown that a synthetic trivalent glycocluster, triacedimannose (TADM), suppresses the Th2\u2010type allergic inflammation. The aim of this study was to compare TADM with two well\u2010known adjuvants, unmethylated cytosine\u2010phosphate\u2010guanine oligodeoxynucleotide (CpG) and monophosphoryl lipid\u00a0A (MPLA) in a grass allergen\u2010induced chronic allergic inflammation model in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Female BALB\/c mice were intranasally sensitized with 50\u00a0\u03bcL of timothy grass pollen extract (TE) twice a week for a period of 15\u00a0weeks. Therapeutic intranasal treatments were then performed once a week after the tenth intranasal TE instillation using TADM (10 or 25\u00a0\u03bcg\/50\u00a0\u03bcL), CpG\u2010ODN (20\u00a0\u03bcg\/50\u00a0\u03bcL) or MPLA (2\u00a0\u03bcg\/50\u00a0\u03bcL). Groups of 9\u201010 animals per treatment were killed 24\u00a0hours after the last timothy dosage. Blood, bronchoalveolar lavage (BAL) fluids and lung biopsies were taken for subsequent analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When mice were repeatedly exposed to TE for 15\u00a0weeks, the number of eosinophils and lymphocytes increased in the BAL fluids. The eosinophil and lymphocyte counts decreased dose\u2010dependently and were practically abolished in the mice treated with TADM. Treatments with MPLA or CpG significantly increased the number<jats:styled-content>s<\/jats:styled-content> of neutrophils, while CpG nonsignificantly decreased eosinophilia compared to timothy exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A novel synthetic glycocluster molecule inhibited the development of grass\u2010induced eosinophilic pulmonary inflammation in mice when administrated in the airways. This compound could be a candidate to be used either as an adjuvant in SIT or as a topical anti\u2010inflammatory treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13419","type":"journal-article","created":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T14:17:32Z","timestamp":1516976252000},"page":"1700-1706","source":"Crossref","is-referenced-by-count":8,"title":["A novel glycocluster molecule prevents timothy\u2010induced allergic airway inflammation in mice"],"prefix":"10.1111","volume":"73","author":[{"given":"M.","family":"Lehto","sequence":"first","affiliation":[{"name":"Department of Occupational Medicine Finnish Institute of Occupational Health Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wolff","sequence":"additional","affiliation":[{"name":"Department of Pathology Finnish Institute of Occupational Health Helsinki 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