{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T03:08:28Z","timestamp":1786504108076,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T00:00:00Z","timestamp":1522108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\/CIRG\/1458\/2016"],"award-info":[{"award-number":["NMRC\/CIRG\/1458\/2016"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81170897"],"award-info":[{"award-number":["81170897"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81441028"],"award-info":[{"award-number":["81441028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The IL<\/jats:styled-content>\u201013 receptor \u03b12 (IL<\/jats:styled-content>\u201013R\u03b12) is a receptor for IL<\/jats:styled-content>\u201013 which has conflicting roles in mediating IL<\/jats:styled-content>\u201013 responses in the lower airway, with little known about its impact on upper airway diseases. We sought to investigate the expression of IL<\/jats:styled-content>\u201013 receptors, IL<\/jats:styled-content>\u201013R\u03b11 and IL<\/jats:styled-content>\u201013R\u03b12, in chronically inflamed nasal epithelium, and explore IL<\/jats:styled-content>\u201013\u2010induced signaling pathways in an in\u00a0vitro model of human nasal epithelial cells (hNEC<\/jats:styled-content>s).<\/jats:p><\/jats:sec>Methods<\/jats:title>The protein and mRNA<\/jats:styled-content> expression levels of IL<\/jats:styled-content>\u201013 and its receptors in nasal biopsies of patients with nasal polyps (NP<\/jats:styled-content>) and healthy controls were evaluated. We investigated goblet cell stimulation with mucus hypersecretion induced by IL<\/jats:styled-content>\u201013 (10\u00a0ng\/mL<\/jats:styled-content>, 72\u00a0hours) treatment in hNEC<\/jats:styled-content>s using a pseudostratified epithelium in air\u2010liquid interface (ALI<\/jats:styled-content>) culture.<\/jats:p><\/jats:sec>Results<\/jats:title>There were significant increases in IL<\/jats:styled-content>\u201013, IL<\/jats:styled-content>\u201013R\u03b11, and IL<\/jats:styled-content>\u201013R\u03b12 mRNA<\/jats:styled-content> and protein levels in NP<\/jats:styled-content> epithelium with healthy controls as baseline. MUC<\/jats:styled-content>5AC mRNA<\/jats:styled-content> positively correlated with IL<\/jats:styled-content>\u201013R\u03b12 (r<\/jats:italic>\u00a0=\u00a0.5886, P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>.002) but not with IL<\/jats:styled-content>\u201013R\u03b11 in primary hNEC<\/jats:styled-content>s. IL<\/jats:styled-content>\u201013 treatment resulted in a significant increase in mRNA<\/jats:styled-content> and protein levels of IL<\/jats:styled-content>\u201013R\u03b12 only in hNEC<\/jats:styled-content>s. IL<\/jats:styled-content>\u201013 treatment induced an activation of extracellular signal\u2010regulated kinases (ERK<\/jats:styled-content>)1\/2 and an upregulation of C\u2010JUN<\/jats:styled-content>, where the IL<\/jats:styled-content>\u201013\u2010induced effects on hNEC<\/jats:styled-content>s could be attenuated by ERK<\/jats:styled-content>1\/2 inhibitor (50\u00a0\u03bcmol\/L) or dexamethasone (10\u22124<\/jats:sup>\u201010\u22127<\/jats:sup>\u00a0mol\/L) treatment.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>IL<\/jats:styled-content>\u201013R\u03b12 has a potential role in IL<\/jats:styled-content>\u201013\u2010induced MUC<\/jats:styled-content>5AC<\/jats:styled-content> and ciliary changes through ERK<\/jats:styled-content>1\/2 signal pathway in the nasal epithelium. IL<\/jats:styled-content>\u201013R\u03b12 may contribute to airway inflammation and aberrant remodeling which are the main pathological features of CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13424","type":"journal-article","created":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T11:36:39Z","timestamp":1517916999000},"page":"1673-1685","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Role of IL<\/scp>\u201013R\u03b12 in modulating IL<\/scp>\u201013\u2010induced MUC<\/scp>5AC<\/scp> and ciliary changes in healthy and CRS<\/scp>wNP<\/scp> mucosa"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7511-0680","authenticated-orcid":false,"given":"J.","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. K.","family":"Andiappan","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network (SIgN) Agency for Science, Technology and Research (A*STAR) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. S.","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. H.","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. T.","family":"Thong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery National University Health System (NUHS) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. K.","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery National University Health System (NUHS) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. G.","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. B.","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The Second Hospital of Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0909-2963","authenticated-orcid":false,"given":"D. Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Yong Loo Lin School of Medicine National University of Singapore Singapore 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