{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T22:28:07Z","timestamp":1773095287801,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T00:00:00Z","timestamp":1542499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670912"],"award-info":[{"award-number":["81670912"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870704"],"award-info":[{"award-number":["81870704"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2019,4]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Hippo\u2010Yes\u2010associated protein (YAP<\/jats:styled-content>) pathway plays an important role in epithelial cell proliferation and development. However, its possible role in chronic rhinosinusitis with nasal polyps (CRS<\/jats:styled-content>wNP<\/jats:styled-content>) remains unknown. We aim to investigate it on nasal epithelial proliferation and remodeling in CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>The expressions of hippo pathway components as well as Ki\u201067 and E\u2010cadherin in the sinonasal mucosa and nasal epithelial cells were analyzed in 14 controls, 14 eosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, and 14 noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>. Nasal epithelial cells from 6 controls, 6 eosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, and 6 noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content> were cultured and treated with lipopolysaccharide (LPS<\/jats:styled-content>), Poly(I:C), or a selective YAP<\/jats:styled-content> inhibitor verteporfin (VP<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>The hippo pathway components MST<\/jats:styled-content>1, LATS<\/jats:styled-content>1\/2, YAP<\/jats:styled-content>, and TEAD<\/jats:styled-content>1 were increased in both eosinophilic and noneosinophilic CRS<\/jats:styled-content>wNP<\/jats:styled-content>, particularly in nasal epithelial cells, along with upregulation of Ki\u201067 and downregulation of E\u2010cadherin. The mRNA<\/jats:styled-content> levels of YAP<\/jats:styled-content> positively correlated with the Ki\u201067 mRNA<\/jats:styled-content> levels, and negatively associated with the E\u2010cadherin mRNA<\/jats:styled-content> levels in polyp tissues and epithelial cells from nasal polyps (NPEC<\/jats:styled-content>s). LPS<\/jats:styled-content> and Poly(I:C) upregulated the YAP<\/jats:styled-content> expression in nasal epithelial cells accompanied by increased TEAD<\/jats:styled-content>1 and Ki\u201067 expression. Conversely, YAP<\/jats:styled-content> inhibition by VP<\/jats:styled-content> decreased TEAD<\/jats:styled-content>1 and Ki\u201067 expression in NPECs.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Hippo pathway components are abnormally upregulated in NPEC<\/jats:styled-content>s, and its effector YAP<\/jats:styled-content> promotes nasal epithelial cells proliferation and remodeling in CRS<\/jats:styled-content>wNP<\/jats:styled-content>. It provides a rationale to explore inhibition of YAP<\/jats:styled-content> as a novel therapeutic strategy for reducing the epithelial proliferation and remodeling in CRS<\/jats:styled-content>wNP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13647","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T12:08:23Z","timestamp":1540555703000},"page":"731-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The hippo pathway effector Yes\u2010associated protein promotes epithelial proliferation and remodeling in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"74","author":[{"given":"Huiyi","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2382-0429","authenticated-orcid":false,"given":"Yueqi","family":"Sun","sequence":"additional","affiliation":[{"name":"Otorhinolaryngology Hospital The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weifeng","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuekun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zijie","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuanggui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Third Affiliated Hospital Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0909-2963","authenticated-orcid":false,"given":"Deyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University Health System National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3377-737X","authenticated-orcid":false,"given":"Qintai","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery The Third Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12503"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.10.009"},{"key":"e_1_2_9_4_1","first-page":"731","article-title":"EPOS 2012: European position paper on rhinosinusitis and nasal polyps 2012. 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