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We determined the genes associated with airway remodeling and eosinophilic inflammation in patients with asthma.<\/jats:p><\/jats:sec>Methods<\/jats:title>We analyzed the transcriptomic data from bronchial biopsies of 81 patients with moderate\u2010to\u2010severe asthma of the U\u2010BIOPRED<\/jats:styled-content> cohort. Expression profiling was performed using Affymetrix arrays on total RNA<\/jats:styled-content>. Transcription binding site analysis used the PRIMA<\/jats:styled-content> algorithm. Localization of proteins was by immunohistochemistry.<\/jats:p><\/jats:sec>Results<\/jats:title>Using stringent false discovery rate analysis, MMP<\/jats:styled-content>\u201010 and MET<\/jats:styled-content> were significantly overexpressed in biopsies with high mucosal eosinophils (HE<\/jats:styled-content>) compared to low mucosal eosinophil (LE<\/jats:styled-content>) numbers. Immunohistochemical analysis confirmed increased expression of MMP<\/jats:styled-content>\u201010 and MET<\/jats:styled-content> in bronchial epithelial cells and in subepithelial inflammatory and resident cells in asthmatic biopsies. Using less\u2010stringent conditions (raw P<\/jats:italic>\u2010value\u00a0<\u00a00.05, log2 fold change\u00a0>\u00a00.5), we defined a 73\u2010gene set characteristic of the HE<\/jats:styled-content> compared to the LE<\/jats:styled-content> group. Thirty\u2010three of 73 genes drove the pathway annotation that included extracellular matrix (ECM<\/jats:styled-content>) organization, mast cell activation, CC<\/jats:styled-content>\u2010chemokine receptor binding, circulating immunoglobulin complex, serine protease inhibitors, and microtubule bundle formation pathways. Genes including MET<\/jats:styled-content> and MMP<\/jats:styled-content>10 involved in ECM<\/jats:styled-content> organization correlated positively with submucosal thickness. Transcription factor binding site analysis identified two transcription factors, ETS<\/jats:styled-content>\u20101 and SOX<\/jats:styled-content> family proteins, that showed positive correlation with MMP<\/jats:styled-content>10 and MET<\/jats:styled-content> expression.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Pathways of airway remodeling and cellular inflammation are associated with submucosal eosinophilia. MET<\/jats:styled-content> and MMP<\/jats:styled-content>\u201010 likely play an important role in these processes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13727","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T02:06:27Z","timestamp":1548295587000},"page":"1102-1112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Contribution of airway eosinophils in airway wall remodeling in asthma: Role of MMP<\/scp>\u201010<\/i> and MET<\/scp><\/i>"],"prefix":"10.1111","volume":"74","author":[{"given":"Chih\u2010Hsi S.","family":"Kuo","sequence":"first","affiliation":[{"name":"Airways Disease National Heart & Lung Institute Imperial College London UK"},{"name":"Biomedical Research Unit Royal Brompton & Harefield NHS Trust London UK"},{"name":"Department of Computing & Data Science Institute Imperial College London 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