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The etiology of allergic sensitization is poorly understood, and its underlying biological mechanisms are not well established. Several studies showed that <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation (<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m) at some CpGs is associated with allergic sensitization. However, no studies have focused on the critical adolescence period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed the association of pre\u2010 and postadolescence genome\u2010wide <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m with allergic sensitization against indoor, outdoor and food allergens, using linear mixed models. We hypothesized that <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m is associated with sensitization in general, and with poly\u2010sensitization status, and these associations are age\u2010 and gender\u2010specific. We tested these hypotheses in the IoW cohort (n\u00a0=\u00a0376) and examined the findings in the <jats:styled-content style=\"fixed-case\">BAMSE<\/jats:styled-content> cohort (n\u00a0=\u00a0267).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Via linear mixed models, we identified 35 CpGs in IoW associated with allergic sensitization (at false discovery rate of 0.05), of which 33 were available in <jats:styled-content style=\"fixed-case\">BAMSE<\/jats:styled-content> and replicated with respect to the direction of associations with allergic sensitization. At the 35 CpGs except for cg19210306 on <jats:italic>C13orf27<\/jats:italic>, a reduction in methylation among atopic subjects was observed, most notably for cg21220721 and cg11699125 (<jats:italic><jats:styled-content style=\"fixed-case\">ACOT<\/jats:styled-content>7<\/jats:italic>). <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m at cg10159529 was strongly correlated with expression of <jats:italic><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>5<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content><\/jats:italic> in peripheral blood (<jats:italic>P<\/jats:italic>\u2010value\u00a0=\u00a06.76\u00a0\u00d7\u00a010<jats:sup>\u221220<\/jats:sup>). Three CpGs (cg14121142, cg23842695, and cg26496795) were identified in IoW with age\u2010specific association between <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m and allergic sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In adolescence, the status of allergic sensitization was associated with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>m differentiation and at some CpGs the association is likely to be age\u2010specific.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13746","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T11:27:43Z","timestamp":1550143663000},"page":"1166-1175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["<scp>DNA<\/scp> methylation and allergic sensitizations: A genome\u2010scale longitudinal study during adolescence"],"prefix":"10.1111","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3557-0364","authenticated-orcid":false,"given":"Hongmei","family":"Zhang","sequence":"first","affiliation":[{"name":"Division of Epidemiology, Biostatistics, and Environmental Health Sciences School of Public Health University of Memphis Memphis TN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhilesh","family":"Kaushal","sequence":"additional","affiliation":[{"name":"Center for Precision Environmental Health Baylor College of Medicine Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon Kebede","family":"Merid","sequence":"additional","affiliation":[{"name":"Institute of Environmental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Mel\u00e9n","sequence":"additional","affiliation":[{"name":"Institute of Environmental Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Sachs' Children's Hospital Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6ran","family":"Pershagen","sequence":"additional","affiliation":[{"name":"Institute of Environmental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal I.","family":"Rezwan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Clinical and Experimental Sciences University of Southampton Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luhang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Mathematical Sciences University of Memphis Memphis Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Ewart","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Michigan State University East Lansing Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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