{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T16:19:44Z","timestamp":1784045984405,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Many skin and mucosal inflammatory disorders, such as atopic dermatitis, have been associated with an impaired epithelial barrier function, which allows allergens, pollutants, or microbes to enter the tissue and activate the immune response. The aim of this study was to establish a method to directly assess in vivo the epidermal barrier function by electrical impedance (EI) spectroscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice epidermal barrier was damaged by epicutaneous application of proteases and cholera toxin and by tape stripping. EI and transepidermal water loss (TEWL) were measured before and after the application. Additionally, histological analysis, immunofluorescence staining, and RT\u2010PCR were performed on skin biopsies to evaluate the epithelial barrier.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A few hours after papain application, a dose\u2010dependent reduction of EI was detected, reflecting the decreased barrier function. At the same time, an increase of TEWL was observed, with a significant negative correlation with EI, demonstrating that EI changes were directly linked to barrier defects. Twenty\u2010four and 48\u00a0hours after the treatment, EI starts to increase to background levels, indicating tissue healing and restoration of skin barrier. Barrier disruption was confirmed by histological analysis showing an impaired stratum corneum and higher cellular infiltration after papain application. In addition, immunofluorescence staining and RT\u2010PCR showed downregulation of molecules involved in the barrier function, such as filaggrin, occludin, and claudin\u20101, and mRNA levels of filaggrin, loricrin, and involucrin. Comparable results were observed after tape stripping and cholera toxin treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Electrical impedance spectroscopy is a rapid and reliable diagnostic tool to detect skin barrier defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.13824","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T07:18:14Z","timestamp":1555399094000},"page":"1934-1944","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Direct assessment of skin epithelial barrier by electrical impedance spectroscopy"],"prefix":"10.1111","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8967-3866","authenticated-orcid":false,"given":"Arturo O.","family":"Rinaldi","sequence":"first","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) Davos Switzerland"},{"name":"Christine K\u00fchne \u2013 Center for Allergy 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