{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T03:41:57Z","timestamp":1788838917161,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T00:00:00Z","timestamp":1573516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["105\u20102628\u2010B\u2010001\u2010009\u2010MY3"],"award-info":[{"award-number":["105\u20102628\u2010B\u2010001\u2010009\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Respiratory syncytial virus (RSV) infection is epidemiologically linked to asthma. During RSV infection, IL\u201033 is elevated and promotes immune cell activation, leading to the development of asthma. However, which immune cells are responsible for triggering airway hyperreactivity (AHR), inflammation and eosinophilia remained to be clarified. We aimed to elucidate the individual roles of IL\u201033\u2010activated innate immune cells, including ILC2s and ST2<jats:sup>+<\/jats:sup> myeloid cells, in RSV infection\u2010triggered pathophysiology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The role of IL\u201033\/ILC2 axis in RSV\u2010induced AHR inflammation and eosinophilia were evaluated in the IL\u201033\u2010deficient and YetCre\u201013 Rosa\u2010DTA mice. Myeloid\u2010specific, IL\u201033\u2010deficient or ST2\u2010deficient mice were employed to examine the role of IL\u201033 and ST2 signaling in myeloid cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that IL\u201033\u2010activated ILC2s were crucial for the development of AHR and airway inflammation, during RSV infection. ILC2\u2010derived IL\u201013 was sufficient for RSV\u2010driven AHR, since reconstitution of wild\u2010type ILC2 rescued RSV\u2010driven AHR in IL\u201013\u2010deficient mice. Meanwhile, myeloid cell\u2010derived IL\u201033 was required for airway inflammation, ST2<jats:sup>+<\/jats:sup> myeloid cells contributed to exacerbation of airway inflammation, suggesting the importance of IL\u201033 signaling in these cells. Local and peripheral eosinophilia is linked to both ILC2 and myeloid IL\u201033 signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study highlights the importance of IL\u201033\u2010activated ILC2s in mediating RSV\u2010triggered AHR and eosinophilia. In addition, IL\u201033 signaling in myeloid cells is crucial for airway inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.14091","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T18:44:06Z","timestamp":1571337846000},"page":"818-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Pulmonary IL\u201033 orchestrates innate immune cells to mediate respiratory syncytial virus\u2010evoked airway hyperreactivity and eosinophilia"],"prefix":"10.1111","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4385-2532","authenticated-orcid":false,"given":"Yi\u2010Hsiu","family":"Wu","sequence":"first","affiliation":[{"name":"Taiwan International Graduate Program in Molecular Medicine National Yang\u2010Ming University and Academia Sinica Taipei Taiwan"},{"name":"Institute of Biomedical Sciences Academia Sinica Taipei 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