{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:43:51Z","timestamp":1787013831656,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T00:00:00Z","timestamp":1575331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There is mounting evidence that systemic uptake of food allergens is key to triggering anaphylaxis. However, direct proof for this theory is still lacking. The purpose of this study was to quantify the absorption and to determine the absorption kinetics of immunoreactive peanut protein in relation to the allergic response in human.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Quantitative protein assays including mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h 2 absorption in human serum. The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics of immunologically intact peanut proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The protein assays worked but were not sensitive enough to trace the minute amounts of absorbed Ara h 2 in human serum. The level of Ara h 6 in serum was found to be up to 0.2\u00a0ng\/mL, but Ara h 2 could not be detected with the ELISA. Both the in vivo and the in vitro methods were successful in demonstrating that: immunoreactive peanut protein was absorbed shortly after ingestion (\u22645\u00a0minutes); the peanut protein concentration peaks between 1 and 4\u00a0hours; and peanut proteins can circulate for at least 48\u00a0hours in the bloodstream.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ingested peanut protein is absorbed systemically and retains its immunoreactive capacity in human serum. However, the precise quantities and the implication for the elicitation of anaphylaxis remains to be elucidated.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.14109","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T14:53:39Z","timestamp":1573570419000},"page":"1721-1729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The quest for ingested peanut protein in human serum"],"prefix":"10.1111","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9471-6234","authenticated-orcid":false,"given":"Anja","family":"Pahlow Mose","sequence":"first","affiliation":[{"name":"Odense Research Center for Anaphylaxis (ORCA) Odense University Hospital Odense Denmark"},{"name":"Department of Dermatology and Allergy Center Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4381-1546","authenticated-orcid":false,"given":"Ejvind","family":"Mortz","sequence":"additional","affiliation":[{"name":"Alphalyse A\/S Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-4549","authenticated-orcid":false,"given":"Per","family":"Stahl Skov","sequence":"additional","affiliation":[{"name":"Odense Research Center for Anaphylaxis (ORCA) Odense University Hospital Odense Denmark"},{"name":"RefLab Aps. Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-0829","authenticated-orcid":false,"given":"Charlotte Gotthard","family":"Mortz","sequence":"additional","affiliation":[{"name":"Odense Research Center for Anaphylaxis (ORCA) Odense University Hospital Odense Denmark"},{"name":"Department of Dermatology and Allergy Center Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-3046","authenticated-orcid":false,"given":"Esben","family":"Eller","sequence":"additional","affiliation":[{"name":"Odense Research Center for Anaphylaxis (ORCA) Odense University Hospital Odense Denmark"},{"name":"Department of Dermatology and Allergy Center Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-5054","authenticated-orcid":false,"given":"Ulrik","family":"Sprog\u00f8e","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6968-148X","authenticated-orcid":false,"given":"Torben","family":"Barington","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8940-038X","authenticated-orcid":false,"given":"Carsten","family":"Bindslev\u2010Jensen","sequence":"additional","affiliation":[{"name":"Odense Research Center for Anaphylaxis (ORCA) Odense University Hospital Odense Denmark"},{"name":"Department of Dermatology and Allergy Center Odense University Hospital Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90319-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.12.1040"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1990.tb00530.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1985.tb01862.x"},{"key":"e_1_2_9_6_1","first-page":"99","article-title":"Passage of intact peptides across the intestine","volume":"65","author":"Gardner ML","year":"1987","journal-title":"Adv Biosci"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2016.11.002"},{"issue":"1","key":"e_1_2_9_8_1","first-page":"G139","article-title":"Intestinal absorption of undegraded proteins in men: presence of bromelain in plasma after oral intake","volume":"273","author":"Castell JV","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1101692"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.03.027"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02999161"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1928.00130200024002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.10.039"},{"key":"e_1_2_9_14_1","first-page":"160","article-title":"Studien \u00fcber uberempfindlicht","volume":"86","author":"Prausnitz C","year":"1921","journal-title":"Centralb Baketerial 1 Abt Orig"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12075"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000321108"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02764.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2012.11.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1096-1269"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.2.882"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201000011"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29249"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.9998"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056002132.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi400110b"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0685-7_4"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400270"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.06.033"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443274"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.11.034"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70203-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199208063270603"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000479388"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fall.14109","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.14109","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/all.14109","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.14109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T18:18:58Z","timestamp":1692814738000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.14109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,3]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1111\/all.14109"],"URL":"https:\/\/doi.org\/10.1111\/all.14109","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,3]]},"assertion":[{"value":"2019-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}