{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T02:51:30Z","timestamp":1768272690644,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T00:00:00Z","timestamp":1583798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI120526"],"award-info":[{"award-number":["R01 AI120526"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early\u2010life wheezing\u2010associated respiratory infection with human rhinovirus (RV) is associated with asthma development. RV infection of 6\u2010day\u2010old immature mice causes mucous metaplasia and airway hyperresponsiveness which is associated with the expansion of IL\u201013\u2010producing type 2 innate lymphoid cells (ILC2s) and dependent on IL\u201025 and IL\u201033. We examined regulation of this asthma\u2010like phenotype by IL\u20101\u03b2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six\u2010day\u2010old wild\u2010type or NRLP3\u2212\/\u2212 mice were inoculated with sham or RV\u2010A1B. Selected mice were treated with IL\u20101 receptor antagonist (IL\u20101RA), anti\u2010IL\u20101\u03b2, or recombinant IL\u20101\u03b2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Rhinovirus infection induced <jats:italic>Il25<\/jats:italic>, <jats:italic>Il33<\/jats:italic>, <jats:italic>Il4<\/jats:italic>, <jats:italic>Il5<\/jats:italic>, <jats:italic>Il13<\/jats:italic>, <jats:italic>muc5ac,<\/jats:italic> and <jats:italic>gob5<\/jats:italic> mRNA expression, ILC2 expansion, mucus metaplasia, and airway hyperresponsiveness. RV also induced lung mRNA and protein expression of pro\u2010IL\u20101\u03b2 and NLRP3 as well as cleavage of caspase\u20101 and pro\u2010IL\u20101\u03b2, indicating inflammasome priming and activation. Lung macrophages were a major source of IL\u20101\u03b2. Inhibition of IL\u20101\u03b2 signaling with IL\u20101RA, anti\u2010IL\u20101\u03b2, or NLRP3 KO increased RV\u2010induced type 2 cytokine immune responses, ILC2 number, and mucus metaplasia, while decreasing IL\u201017 mRNA expression. Treatment with IL\u20101\u03b2 had the opposite effect, decreasing IL\u201025, IL\u201033, and mucous metaplasia while increasing IL\u201017 expression. IL\u20101\u03b2 and IL\u201017 each suppressed <jats:italic>Il25<\/jats:italic>, <jats:italic>Il33,<\/jats:italic> and <jats:italic>muc5ac<\/jats:italic> mRNA expression in cultured airway epithelial cells. Finally, RV\u2010infected 6\u2010day\u2010old mice showed reduced IL\u20101\u03b2 mRNA and protein expression compared to mature mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Macrophage IL\u20101\u03b2 limits type 2 inflammation and mucous metaplasia following RV infection by suppressing epithelial cell innate cytokine expression. Reduced IL\u20101\u03b2 production in immature animals provides a mechanism permitting asthma development after early\u2010life viral infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.14241","type":"journal-article","created":{"date-parts":[[2020,2,22]],"date-time":"2020-02-22T08:15:30Z","timestamp":1582359330000},"page":"2005-2019","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["IL\u20101\u03b2 prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early\u2010life rhinovirus infection in mice"],"prefix":"10.1111","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2970-6715","authenticated-orcid":false,"given":"Mingyuan","family":"Han","sequence":"first","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer R.","family":"Bermick","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charu","family":"Rajput","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Lei","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9772-1255","authenticated-orcid":false,"given":"Adam M.","family":"Goldsmith","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin R.","family":"Jarman","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Lee","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics University of Michigan Medical School Ann Arbor Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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