{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:43:43Z","timestamp":1790775823018,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["NUCATS U19 069526"],"award-info":[{"award-number":["NUCATS U19 069526"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Skin biopsies promote our understanding of atopic dermatitis\/AD pathomechanisms in infants\/toddlers with early\u2010onset AD, but are not feasible in pediatric populations. Tape strips are an emerging, minimally invasive alternative, but global transcriptomic profiling in early pediatric AD is lacking. We aimed to provide global lesional and nonlesional skin profiles of infants\/toddlers with recent\u2010onset, moderate\u2010to\u2010severe AD using tape strips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixteen tape strips were collected for RNA\u2010seq profiling from 19 infants\/toddlers (&lt;5\u00a0years old; lesional and nonlesional) with early\u2010onset moderate\u2010to\u2010severe AD (\u22646\u00a0months) and 17 healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 1829 differentially expressed genes\/DEGs in lesional AD and 662 DEGs in nonlesional AD, vs healthy skin (fold\u2010change \u22652, FDR &lt;0.05), with 100% sample recovery. Both lesional and nonlesional skin showed significant dysregulations of Th2 (CCL17 and IL4R) and Th22\/Th17 (IL36G, CCL20, and S100As)\u2010related genes, largely lacking significant Th1\u2010skewing. Significant down\u2010regulation of terminal differentiation (FLG and FLG2), lipid synthesis\/metabolism (ELOVL3 and FA2H), and tight junction (CLDN8) genes were primarily seen in lesional AD. Significant negative correlations were identified between Th2 measures and epidermal barrier gene\u2010subsets and individual genes (FLG with IL\u20104R and CCL17; <jats:italic>r\u00a0<\/jats:italic>&lt;\u00a0\u22120.4, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Significant correlations were also identified between clinical measures (body surface area\/BSA, pruritus ADQ, and transepidermal water loss\/TEWL) with immune and barrier mRNAs in lesional and\/or nonlesional AD (FLG\/FLG2 with TEWL; <jats:italic>r\u00a0<\/jats:italic>&lt;\u00a0\u22120.4, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>RNA\u2010seq profiling using tape strips in early\u2010onset pediatric AD captures immune and barrier alterations in both lesional and nonlesional skin. Tape strips provide insight into disease pathomechanisms and cutaneous disease activity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.14490","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T15:39:30Z","timestamp":1594222770000},"page":"314-325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Tape strips from early\u2010onset pediatric atopic dermatitis highlight disease abnormalities in nonlesional skin"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0497-8351","authenticated-orcid":false,"given":"Ana B.","family":"Pavel","sequence":"first","affiliation":[{"name":"Laboratory of Inflammatory Skin Diseases, Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"},{"name":"Department of Biomedical Engineering University of Mississippi MS 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Diseases, Department of Dermatology Icahn School of Medicine at Mount Sinai New York NY USA"},{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"},{"name":"Department of Dermatology University of Rome Tor Vergata Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisleen","family":"Diaz","sequence":"additional","affiliation":[{"name":"Laboratory for Investigative Dermatology The Rockefeller University New York NY USA"},{"name":"Ponce Health Sciences University School of Medicine Ponce PR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Lefferdink","sequence":"additional","affiliation":[{"name":"Department of Dermatology Northwestern University Feinberg School of Medicine Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milie M.","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Northwestern University Feinberg 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