{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:08:22Z","timestamp":1790345302978,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T00:00:00Z","timestamp":1635811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The specificities of IgE and IgG for allergen molecules in patients with inborn errors of immunity (IEI) have not been investigated in detail.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study IgE and IgG antibody specificities in patients with defined hyper\u2010IgE syndromes (HIES) using a comprehensive panel of allergen molecules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used chips containing micro\u2010arrayed allergen molecules to analyze allergen\u2010specific IgE and IgG levels in sera from two groups of HIES patients: Autosomal recessive mutations in phosphoglucomutase\u20103 (PGM3); Autosomal dominant negative mutations of STAT3 (STAT3); and age\u2010matched subjects with allergic sensitizations. Assays with rat basophil leukemia cells transfected with human Fc\u03b5RI were performed to study the biological relevance of IgE sensitizations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Median total IgE levels were significantly lower in the sensitized control group (212.9\u00a0kU\/L) as compared to PGM3 (5042\u00a0kU\/L) and STAT3 patients (2561\u00a0kU\/L). However, PGM3 patients had significantly higher allergen\u2010specific IgE levels and were sensitized to a larger number of allergen molecules as compared to STAT3 patients. Biological relevance of IgE sensitization was confirmed for PGM3 patients by basophil activation testing. PGM3 patients showed significantly lower cumulative allergen\u2010specific IgG responses in particular to milk and egg allergens as compared to STAT3 patients and sensitized controls whereas total IgG levels were comparable to STAT3 patients and significantly higher than in controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The analysis with multiple micro\u2010arrayed allergen molecules reveals profound differences of allergen\u2010specific IgE and IgG recognition in PGM3 and STAT3 patients which may be useful for classification of IEI and clinical characterization of patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15143","type":"journal-article","created":{"date-parts":[[2021,10,18]],"date-time":"2021-10-18T06:04:39Z","timestamp":1634537079000},"page":"1761-1771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Profound differences in IgE and IgG recognition of micro\u2010arrayed allergens in hyper\u2010IgE syndromes"],"prefix":"10.1111","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3855-217X","authenticated-orcid":false,"given":"Victoria","family":"Garib","sequence":"first","affiliation":[{"name":"Division of Immunopathology Department of Pathophysiology and Allergy Research Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna  Wien Austria"},{"name":"Ministry of Innovation Development  Tashkent Uzbekistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Ben\u2010Ali","sequence":"additional","affiliation":[{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02) Department of Immunology Institut Pasteur de Tunis and University Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kundi","sequence":"additional","affiliation":[{"name":"Department for Environmental Health Center for Public Health Medical University Vienna  Wien Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2575-4127","authenticated-orcid":false,"given":"Mirela","family":"Curin","sequence":"additional","affiliation":[{"name":"Division of Immunopathology Department of Pathophysiology and Allergy Research Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna  Wien Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roukaya","family":"Yaakoubi","sequence":"additional","affiliation":[{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02) Department of Immunology Institut Pasteur de Tunis and University Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imen","family":"Ben\u2010Mustapha","sequence":"additional","affiliation":[{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02) Department of Immunology Institut Pasteur de Tunis and University Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najla","family":"Mekki","sequence":"additional","affiliation":[{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02) Department of Immunology Institut Pasteur de Tunis and University Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renate","family":"Froeschl","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Perkmann","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-3365","authenticated-orcid":false,"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[{"name":"Division of Immunopathology Department of Pathophysiology and Allergy Research Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna  Wien Austria"},{"name":"NRC Institute of Immunology FMBA of Russia  Moscow Russia"},{"name":"Laboratory of Immunopathology Department of Clinical Immunology and Allergology Sechenov First Moscow State Medical University  Moscow Russia"},{"name":"Karl Landsteiner University of Health Sciences  Krems Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed\u2010Ridha","family":"Barbouche","sequence":"additional","affiliation":[{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LR11IPT02) Department of Immunology Institut Pasteur de Tunis and University Tunis El Manar  Tunis Tunisia"},{"name":"Medical School University of Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.10.023"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ai.2018.03.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20172306"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.waojou.2021.100513"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13578"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-021-01041-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-019-00737-x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(66)90119-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.97.1.75"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199903043400904"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06096"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa073687"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc076367"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-021-01051-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.04.020"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.02.025"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.06.248"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.12.987"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-015-0574-5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.08.045"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13969"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12416"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0711fje"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.10.008"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.05.023"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12366"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2011.04492.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.95.08030483"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02363.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.03.029"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1398-9995.2003.00335.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70195-8"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12512"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.08.042"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.11.051"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.02.034"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13260"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.14.1559"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.06.025"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.15143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/all.15143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.15143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T01:53:51Z","timestamp":1692842031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.15143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,2]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/all.15143"],"URL":"https:\/\/doi.org\/10.1111\/all.15143","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,2]]},"assertion":[{"value":"2021-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}