{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:22:35Z","timestamp":1788261755283,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,10,30]],"date-time":"2021-10-30T00:00:00Z","timestamp":1635552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The shrimp <jats:italic>Litopenaeus vannamei<\/jats:italic> is an important source of food allergens but its allergenic repertoire is poorly characterized. Cross\u2010reactivity between crustacean and mites has been reported, with tropomyosin, the most relevant allergen involved. The aim of this study was to investigate the structural and immunological properties of a recombinant Fatty Acid Binding Protein (FABP) family from <jats:italic>L<\/jats:italic>. <jats:italic>vannamei<\/jats:italic> (LvFABP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ELISA, skin prick test (SPT) and basophil activation assays were performed to determine IgE reactivity and allergenic activity of LvFABP. LC\u2010MS\/MS and Circular Dichroism experiments were done for structural analysis. B\u2010cell epitope mapping with overlapping peptides, and cross\u2010inhibition studies using human sera were done to identify antigenic regions and cross\u2010reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The recombinant LvFABP bound serum IgE from 27% of 36\u00a0shrimp allergic patients and showed allergenic activity when tested for basophil activation and SPT in a selected number of them. CD\u2010spectroscopy of LvFABP revealed that the protein is folded with a secondary structure composed of mainly \u03b2\u2010strands and a smaller fraction of \u03b1 helices. This is consistent with molecular modelling results, which exhibit a typical \u03b2 barrel fold with two \u03b1\u2010helices and ten \u03b2\u2010strands. Epitope mapping identified two IgE\u2010binding antigenic regions and inhibition assays found high cross\u2010reactivity between LvFABP and Blo t 13, mediated by the antigenic region involving amino acids 54 to 72.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results show that LvFABP is a shrimp allergen that cross reacts with the house dust mite allergen Blo t 13 and has allergenic activity, which suggest that it could be clinically relevant in case of shellfish allergy. This new allergen, named Lit v 13, will also help to understand basic mechanisms of sensitization to shrimp.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15154","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T17:08:20Z","timestamp":1635181700000},"page":"1534-1544","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Structural and allergenic properties of the fatty acid binding protein from shrimp <i>Litopenaeus vannamei<\/i>"],"prefix":"10.1111","volume":"77","author":[{"given":"Marlon","family":"M\u00fanera","sequence":"first","affiliation":[{"name":"Institute for Immunological Research University of Cartagena  Cartagena Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalgys","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Institute for Immunological Research University of Cartagena  Cartagena Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Wortmann","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, BioTechMed Graz University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6007-5376","authenticated-orcid":false,"given":"Josefina","family":"Zakzuk","sequence":"additional","affiliation":[{"name":"Institute for Immunological Research University of Cartagena  Cartagena Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2261-958X","authenticated-orcid":false,"given":"Walter","family":"Keller","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biosciences, BioTechMed Graz University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Caraballo","sequence":"additional","affiliation":[{"name":"Institute for Immunological Research University of Cartagena  Cartagena Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2246-9697","authenticated-orcid":false,"given":"Leonardo","family":"Puerta","sequence":"additional","affiliation":[{"name":"Institute for Immunological Research University of Cartagena  Cartagena 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