{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T07:28:21Z","timestamp":1780990101118,"version":"3.54.1"},"reference-count":81,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T00:00:00Z","timestamp":1638489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI080581"],"award-info":[{"award-number":["R01 AI080581"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>IL\u20105\u2010dependent residential and IL\u201018\u2010transformed pathogenic eosinophils have been reported; however, the role of IL\u201018\u2010transformed CD274\u2010expressing pathogenic eosinophils compared to IL\u20105\u2010generated eosinophils in promoting airway obstruction in asthma has not yet been examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eosinophils are detected by tissue anti\u2010MBP and anti\u2010EPX immunostaining, CD274 expression by flow cytometry, and airway resistance using the Buxco FinePointe RC system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic>\u2010challenged wild\u2010type mice, and different gene\u2010deficient mice including na\u00efve CC10\u2010IL\u201018\u2010transgenic mice, accumulate mostly peribronchial and perivascular CD274\u2010expressing eosinophils except na\u00efve CD2\u2010IL\u20105\u2010transgenic mice. Additionally, we show that CD2\u2010IL\u20105 transgenic\u00a0mice following rIL\u201018 treatment accumulate high number of CD274\u2010expressing perivascular and peribronchial eosinophils with induced collagen, goblet cell hyperplasia and airway resistance compared to saline\u2010challenged CD2\u2010IL5\u00a0transgenic mice. Furthermore, we also show that even <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic>\u2010challenged <jats:italic>IL<\/jats:italic>\u2010<jats:italic>5<\/jats:italic>\n<jats:sup>\u2212\/\u2212<\/jats:sup> mice and rIL\u201018\u00a0given <jats:italic>\u0394dblGATA<\/jats:italic> mice accumulate CD274\u2010expressing eosinophil\u2010associated asthma pathogenesis including airway obstruction. Most importantly, we provide evidence that neutralization of CD274 and IL\u201018 in <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic>\u2010challenged mice ameliorate experimental asthma. Taken together, the data presented are clinically significant in establishing that anti\u2010IL\u201018 neutralization is a novel immunotherapy to restrict asthma pathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We demonstrate that IL\u201018 is critical for inducing asthma pathogenesis, and neutralization of CD274 is a potential immunotherapeutic strategy for asthma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15180","type":"journal-article","created":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T15:19:15Z","timestamp":1637421555000},"page":"1165-1179","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Role of IL\u201018\u2010transformed CD274\u2010expressing eosinophils in promoting airway obstruction in experimental asthma"],"prefix":"10.1111","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4266-4684","authenticated-orcid":false,"given":"Anil","family":"Mishra","sequence":"first","affiliation":[{"name":"John W. Deming Department of Medicine Tulane Eosinophilic Disorders Center (TEDC) Section of Pulmonary Diseases Tulane University School of Medicine  New Orleans Louisina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewan","family":"Majid","sequence":"additional","affiliation":[{"name":"John W. Deming Department of Medicine Tulane Eosinophilic Disorders Center (TEDC) Section of Pulmonary Diseases Tulane University School of Medicine  New Orleans Louisina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemanth Kumar","family":"Kandikattu","sequence":"additional","affiliation":[{"name":"John W. Deming Department of Medicine Tulane Eosinophilic Disorders Center (TEDC) Section of Pulmonary Diseases Tulane University School of Medicine  New Orleans Louisina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7776-1543","authenticated-orcid":false,"given":"Chandra Sekhar","family":"Yadavalli","sequence":"additional","affiliation":[{"name":"John W. Deming Department of Medicine Tulane Eosinophilic Disorders Center (TEDC) Section of Pulmonary Diseases Tulane University School of Medicine  New Orleans Louisina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathisha","family":"Upparahalli Venkateshaiah","sequence":"additional","affiliation":[{"name":"John W. Deming Department of Medicine Tulane Eosinophilic Disorders Center (TEDC) Section of Pulmonary Diseases Tulane University School of Medicine  New Orleans Louisina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118296"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.supplement_2.13tac170"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.161.5.9903102"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/86365"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10224"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0110025"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2020.07.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104716"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3341"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.128"},{"issue":"11","key":"e_1_2_9_12_1","first-page":"844","article-title":"Significance of mouse models in dessecting the mechanism of human eosinophilic gastrointestinal diseases (EGID)","volume":"2","author":"Mishra A","year":"2013","journal-title":"J Gastroenterol Hepatol Res"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.12.6681"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110424200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00141.2014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6560"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gastro.2003.07.007"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100283"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19133"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17974"},{"issue":"57","key":"e_1_2_9_21_1","first-page":"137","article-title":"Eosinophil activation and function in health and disease","volume":"1992","author":"Abu\u2010Ghazaleh RI","year":"1992","journal-title":"Immunol Ser"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.3.1332"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.02.011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761997000800010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(02)02302-X"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200208-789OC"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00117-2020"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201611-2234OC"},{"issue":"1","key":"e_1_2_9_29_1","first-page":"33","article-title":"Interleukin 13 and 18 serum concentration in children with cow milk hypersensitivity","volume":"8","author":"Bala G","year":"2004","journal-title":"Med Wieku Rozwoj"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mpg.0000233160.35786.35"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.102.1.e6"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00000233"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2012.0029"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054623"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02458.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2009.112"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/cti.2013.13"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2464"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2015.30"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13051"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.4.785"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00223.2011"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1095-4_21"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1004159"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.05.025"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.02.004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxm057"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2000.0799"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxl021"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706378104"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607863103"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.02.027"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.076"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)07302-9"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.116436"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.06.009"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.F251"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03119.x"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-009-0081-7"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.05.005"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.10.008"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2011.01974.x"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.04.047"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1159\/000323433"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324151"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2001.790114.x"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8608886"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02302.x"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.5.691"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI85664"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3181a33649"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201108-1545OC"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-007-0506-2"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090847"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931259100"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20022235"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535458"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2013.09.004"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00033811"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.55.105"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1159\/000452654"},{"key":"e_1_2_9_82_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/8085615","article-title":"Elevated levels of activated and pathogenic eosinophils characterize moderate\u2010severe house dust mite allergic rhinitis","volume":"2020","author":"Chen Y","year":"2020","journal-title":"J Immunol Res"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.15180","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/all.15180","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.15180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T16:06:30Z","timestamp":1692979590000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.15180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,3]]},"references-count":81,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/all.15180"],"URL":"https:\/\/doi.org\/10.1111\/all.15180","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,3]]},"assertion":[{"value":"2021-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}