{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:02:18Z","timestamp":1791453738984,"version":"4.3.3"},"reference-count":61,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T00:00:00Z","timestamp":1647216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005624","name":"Medizinischen Hochschule Hannover","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Atopic dermatitis (AD) and psoriasis represent two of the most common inflammatory skin diseases in developed countries. A hallmark of both diseases is T\u2010cell infiltration into the skin. However, it is still not clarified to what extent these infiltrating T cells are antigen\u2010specific skin\u2010homing T cells or unspecific heterogeneous bystander cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To elucidate this, T cells from lesional skin and from blood of 9 AD and 10 psoriasis patients were compared by receptor (TCR) sequencing. Therefore, peripheral blood mononuclear cells (PBMC) were cell\u2010sorted according to expression of the cutaneous leukocyte antigen (CLA) into skin\u2010homing (CLA\n                      <jats:sup>+<\/jats:sup>\n                      ) and non\u2010skin\u2010homing (CLA\n                      <jats:sup>\u2212<\/jats:sup>\n                      ) subfractions. Aeroallergen\u2010specific T\u2010cell lines were grown from AD patients\u2019 PBMC in parallel.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intra\u2010individual comparison of TCRB CDR3 regions revealed that clonally expanded T cells in skin lesions of both AD and psoriasis patients corresponded to skin\u2010homing circulating T cells. However, in psoriasis patients, these T\u2010cell clones were also detectable to a larger extent among CLA\n                      <jats:sup>\u2212<\/jats:sup>\n                      circulating T cells. Up to 28% of infiltrating cells in AD skin were identified as allergen\u2010specific by overlapping TCR sequences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data show that in line with the systemic nature of psoriasis, T\u2010cell clones that infiltrate psoriatic skin lesions do not exclusively possess skin\u2010homing ability and are therefore most probably specific to antigens that are not exclusively expressed or located in the skin. T cells driving AD skin inflammation appear to home nearly exclusively to the skin and are, to a certain extent, specific to aeroallergens.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.15272","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T14:39:20Z","timestamp":1646663960000},"page":"2737-2747","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["T\u2010cell receptor sequencing specifies psoriasis as a systemic and atopic dermatitis as a skin\u2010focused, allergen\u2010driven disease"],"prefix":"10.1111","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6651-0458","authenticated-orcid":false,"given":"Lennart M.","family":"Roesner","sequence":"first","affiliation":[{"name":"Division of Immunodermatology and Allergy Research Department of Dermatology and Allergy Hannover Medical School  Hannover Germany"},{"name":"Cluster of Excellence RESIST (EXC 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