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Associations between individual DNAm at CpGs and allergic diseases have been reported, but their joint predictive capability is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Data were obtained from 240 children of the German LISA cohort. DNAm was measured in blood clots at 6 (<jats:italic>N<\/jats:italic>\u00a0=\u00a0234) and 10\u00a0years (<jats:italic>N<\/jats:italic>\u00a0=\u00a0227) using the Illumina EPIC chip. Presence of aeroallergen sensitization was measured in blood at 6, 10, and 15\u00a0years. We calculated six methylation risk scores (MRS) for allergy\u2010related phenotypes, like total and specific IgE, asthma, or any allergies, based on available publications and assessed their performances both cross\u2010sectionally (biomarker) and prospectively (predictor of the disease). Dose\u2013response associations between aeroallergen sensitization and MRS were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All six allergy\u2010related MRS were highly correlated (<jats:italic>r<\/jats:italic>\u00a0&gt;\u00a0.86), and seven CpGs were included in more than one MRS. Cross\u2010sectionally, we observed an 81% increased risk for aeroallergen sensitization at 6\u00a0years with an increased MRS by one standard deviation (best\u2010performing MRS, 95% confidence interval\u00a0=\u00a0[43%; 227%]). Significant associations were also seen cross\u2010sectionally at 10\u00a0years and prospectively, though the effect of the latter was attenuated when restricted to participants not sensitized at baseline. A clear dose\u2013response relationship with levels of aeroallergen sensitization could be established cross\u2010sectionally, but not prospectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found good classification and prediction capabilities of calculated allergy\u2010related MRS cross\u2010sectionally, underlining the relevance of altered gene\u2010regulation in allergic diseases and providing insights into potential DNAm biomarkers of aeroallergen sensitization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15315","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:44:50Z","timestamp":1650357890000},"page":"2803-2817","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Methylation risk scores for childhood aeroallergen sensitization: Results from the LISA birth 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