{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:06:53Z","timestamp":1790838413549,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["H2020\u2010NMBP\u2010X\u2010KET\u20102017\u2010768641"],"award-info":[{"award-number":["H2020\u2010NMBP\u2010X\u2010KET\u20102017\u2010768641"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diagnosis of food allergies is challenging, as combining information from specific IgE (sIgE)\u2010sensitization pattern and skin prick tests (SPTs) with clinical history is necessary for a personalized management of allergic patients. The aim of this study was to compare two molecular tests, the ImmunoCAP ISAC (ISAC) and the Allergy Explorer, version 2 (ALEX<jats:sup>2<\/jats:sup>) in the context of pollen food syndrome (PFS) diagnosis in a real\u2010life scenario, to assess the benefit of multiplex testing in PFS patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Diagnosis of food allergy was performed in 53 patients. Allergen\u2010sIgE concentrations were measured with ISAC and ALEX<jats:sup>2<\/jats:sup>. Results for sIgE were statistically compared with each other, with SPT results and with clinical presentation of the patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using ISAC as reference test for sIgE measurements, the average sensitivity of ALEX<jats:sup>2<\/jats:sup> for PR\u201010 allergens was 83.2% and the average specificity 88.0%. If only low sIgE concentrations were included, the sensitivity was 60.8% and the specificity 91.1%. Apple and hazelnut sensitizations were confirmed in most patients by concordance of sIgE and SPT results. Significant correlations were shown between clinical symptoms and Mal d 1\u2010 and Gly m 4\u2010sIgE levels measured by both tests and for Cor a 1\u2010sIgE levels measured by ALEX<jats:sup>2<\/jats:sup>. In eight patients, profilin related symptoms were supported by Hev b 8\u2010sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Multiplex testing is beneficial to understand patient\u2010specific individual sensitization profiles and to providing personalized management recommendations. In the future, custom\u2010designed test kits might enable reducing costs of multiplex testing for specific patient groups without compromising the diagnostic value.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15329","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T14:02:20Z","timestamp":1651240940000},"page":"3028-3040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Real\u2010life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy"],"prefix":"10.1111","volume":"77","author":[{"given":"Luisa","family":"Diem","sequence":"first","affiliation":[{"name":"Institute for Pathophysiology and Allergy Research Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bellinda","family":"Neuherz","sequence":"additional","affiliation":[{"name":"Institute for Pathophysiology and Allergy Research Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Rohrhofer","sequence":"additional","affiliation":[{"name":"Institute for Pathophysiology and Allergy Research Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9974-7929","authenticated-orcid":false,"given":"Larissa","family":"Koidl","sequence":"additional","affiliation":[{"name":"Institute for Pathophysiology and Allergy Research Center for Pathophysiology, Infectiology and Immunology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8277-1700","authenticated-orcid":false,"given":"Riccardo","family":"Asero","sequence":"additional","affiliation":[{"name":"Ambulatorio di Allergologia Clinica San Carlo  Paderno Dugnano (MI) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2775-3681","authenticated-orcid":false,"given":"Knut","family":"Brockow","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Biederstein Faculty of Medicine Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1093-3627","authenticated-orcid":false,"given":"Araceli","family":"Diaz Perales","sequence":"additional","affiliation":[{"name":"Centro de Biotecnolog\u00eda Y Gen\u00f3mica de Plantas (CBGP, UPM\u2010INIA) Universidad Polit\u00e9cnica de Madrid (UPM) \u2010Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA)  Madrid Spain"},{"name":"Departamento de Biotecnolog\u00eda\u2010Biolog\u00eda Vegetal Escuela T\u00e9cnica Superior de Ingenier\u00eda Agron\u00f3mica Alimentaria y de Biosistemas. Universidad Polit\u00e9cnica de Madrid (UPM)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaretha","family":"Faber","sequence":"additional","affiliation":[{"name":"Department of Dermatology AZ KLINA  Brasschaat Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Gebhardt","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Biederstein Faculty of Medicine Technical University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5228-471X","authenticated-orcid":false,"given":"Mar\u00eda Jos\u00e9","family":"Torres","sequence":"additional","affiliation":[{"name":"Allergy Unit Hospital Regional Universitario de M\u00e1laga Universidad de M\u00e1laga\u2010IBIMA\u2010BIONAND  M\u00e1laga 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