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Clinical reactivity and presentation, such as thresholds and symptoms, are considered challenging to predict ex vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To identify changes of peripheral immune signatures during oral food challenges (OFC) that correlate with the clinical outcome in patients with peanut allergy (PA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Children with a positive (OFC<jats:sup>+<\/jats:sup>, <jats:italic>n<\/jats:italic>\u00a0=\u00a016) or a negative (OFC<jats:sup>\u2212<\/jats:sup>, <jats:italic>n<\/jats:italic>\u00a0=\u00a010) OFC\u2010outcome were included (controls, <jats:italic>n<\/jats:italic>\u00a0=\u00a07). Single\u2010cell mass cytometry\/unsupervised analysis allowed unbiased immunophenotyping during OFC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Peripheral immune profiles correlated with OFC outcome. OFC<jats:sup>+<\/jats:sup>\u2010profiles revealed mainly decreased Th2 cells, memory Treg and activated NK cells, which had an increased homing marker expression signifying immune cell migration into effector tissues along with symptom onset. OFC<jats:sup>\u2212<\/jats:sup>\u2010profiles had also signs of ongoing inflammation, but with a signature of a controlled response, lacking homing marker expression and featuring a concomitant increase of Th2\u2010shifted CD4<jats:sup>+<\/jats:sup> T cells and Treg cells. Low versus high threshold reactivity\u2010groups had differential frequencies of intermediate monocytes and myeloid dendritic cells at baseline. Low threshold was associated with increased CD8<jats:sup>+<\/jats:sup> T cells and reduced memory cells (central memory [CM] CD4<jats:sup>+<\/jats:sup> [Th2] T cells, CM CD8<jats:sup>+<\/jats:sup> T cells, Treg). Immune signatures also discriminated patients with preferential skin versus gastrointestinal symptoms, whereby skin signs correlated with increased expression of CCR4, a molecule enabling skin trafficking, on various immune cell types.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We showed that peripheral immune signatures reflected dynamics of clinical outcome during OFC with peanut. Those immune alterations hold promise as a basis for predictive OFC biomarker discovery to monitor disease outcome and therapy of PA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15408","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T16:52:47Z","timestamp":1655225567000},"page":"1020-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["High\u2010dimensional immune profiles correlate with phenotypes of peanut allergy during food\u2010allergic reactions"],"prefix":"10.1111","volume":"78","author":[{"given":"Julia","family":"Klueber","sequence":"first","affiliation":[{"name":"Department of Infection and Immunity Luxembourg Institute of Health  Esch\u2010sur\u2010Alzette Luxembourg"},{"name":"Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis University of Southern Denmark  Odense C 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