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The etiology is unknown, however, genetic heritability studies suggest environmental factors play a key role in pathogenesis. Detergents, such as sodium dodecyl sulfate (SDS), are common ingredients in household products such as dish soap and toothpaste. We hypothesized detergent exposure decreases epithelial barrier function and induces esophageal inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Immortalized esophageal epithelial cells (EPC2) were cultured in air\u2010liquid interface (ALI) and exposed to SDS. Barrier function\/activity was assessed by transepithelial electrical resistance (TEER), FITC\u2010dextran flux, and RT\u2010PCR. Additionally, SDS\u2010treated mouse esophageal organoids were evaluated for morphology. To investigate the effects of SDS in vivo, mice were treated with 0.5% SDS in drinking water for 14\u2009days. Esophagi were assessed by gross morphology, histopathology, protein expression, and bulk RNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When EPC2 cells were exposed to SDS (5\u00a0\u03bcg\/ml) for 96\u2009h, TEER decreased (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.03), and FITC\u2010dextran flux increased (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0002). mRNA expression of IL\u201033 increased 4.5\u2010fold (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.02) at 6\u00a0h and DSG1 decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) by 72\u2009h. Disrupted epithelial integrity was noted in SDS\u2010treated esophageal organoids. When mice were exposed to SDS, they showed increased esophageal width, chemokine, and metalloprotease levels. Mice treated with SDS also showed increased IL\u201033 protein expression, basal zone hyperplasia, CD4<jats:sup>+<\/jats:sup> cell infiltration, and esophageal eosinophilia. RNA sequencing revealed upregulation of immune response pathway genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Exposure to SDS decreases esophageal barrier integrity, stimulates IL\u201033 production, and promotes epithelial hyperplasia and tissue eosinophilia. Detergents may be a key environmental trigger in EoE pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15457","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T08:01:33Z","timestamp":1658995293000},"page":"192-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":109,"title":["Detergent exposure induces epithelial barrier dysfunction and eosinophilic inflammation in the esophagus"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9909-0445","authenticated-orcid":false,"given":"Alfred D.","family":"Doyle","sequence":"first","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic Arizona  Scottsdale Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia Y.","family":"Masuda","sequence":"additional","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic Arizona  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