{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:12:33Z","timestamp":1790626353885,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["948207"],"award-info":[{"award-number":["948207"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["948207"],"award-info":[{"award-number":["948207"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pericles.pericles-gcp.literatumonline.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Understanding the complex orchestrated inflammation in atopic dermatitis (AD), one of the most common chronic inflammatory diseases worldwide, is essential for therapeutic approaches. However, a comparative analysis on the single\u2010cell level of the inflammation signatures correlated with the severity is missing so far.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We applied single\u2010cell RNA and T\u2010cell receptor (TCR) sequencing on immune cells enriched from skin biopsies and matched blood samples of AD in comparison with psoriasis (PS) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Clonally propagated skin\u2010derived T cells showed disease\u2010specific TCR motifs shared between patients which was more pronounced in PS compared to AD. The disease\u2010specific T\u2010cell clusters were mostly of a Th2\/Th22 sub\u2010population in AD and Th17\/Tc17 in PS, and their numbers were associated with severity scores in both diseases. Herein, we provide for the first time a list that associates cell type\u2010specific gene expression with the severity of the two most common chronic inflammatory skin diseases. Investigating the cell signatures in the patients\u00b4 PBMCs and skin stromal cells, a systemic involvement of type\u20103 inflammation was clearly detectable in PS circulating cells, while in AD inflammatory signatures were most pronounced in fibroblasts, pericytes, and keratinocytes. Compositional and functional analyses of myeloid cells revealed the activation of antiviral responses in macrophages in association with disease severity in both diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Different disease\u2010driving cell types and subtypes which contribute to the hallmarks of type\u20102 and type\u20103 inflammatory signatures and are associated with disease activities could be identified by single\u2010cell RNA\u2010seq and TCR\u2010seq in AD and PS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.15486","type":"journal-article","created":{"date-parts":[[2022,8,20]],"date-time":"2022-08-20T04:05:39Z","timestamp":1660968339000},"page":"439-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Single\u2010cell profiles reveal distinctive immune response in atopic dermatitis in contrast to psoriasis"],"prefix":"10.1111","volume":"78","author":[{"given":"Bowen","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine (CiiM), a joint venture between the Helmholtz Centre for Infection Research (HZI) Hannover Medical School (MHH)  Hannover Germany"},{"name":"TWINCORE, a joint venture between the Helmholtz\u2010Centre for Infection Research (HZI) and Hannover Medical School (MHH)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6651-0458","authenticated-orcid":false,"given":"Lennart M.","family":"Roesner","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Division of Immunodermatology and Allergy Research Hannover Medical School  Hannover Germany"},{"name":"Cluster of Excellence RESIST (EXC 2155) Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4806-599X","authenticated-orcid":false,"given":"Stephan","family":"Traidl","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Division of Immunodermatology and Allergy Research Hannover Medical School  Hannover Germany"},{"name":"Cluster of Excellence RESIST (EXC 2155) Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie A. C. M.","family":"Koeken","sequence":"additional","affiliation":[{"name":"Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine (CiiM), a joint venture between the Helmholtz Centre for Infection Research (HZI) Hannover Medical School (MHH)  Hannover Germany"},{"name":"TWINCORE, a joint venture between the Helmholtz\u2010Centre for Infection Research (HZI) and Hannover Medical School (MHH)  Hannover Germany"},{"name":"Department of Internal Medicine and Radboud Center for Infectious Diseases Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Jian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine (CiiM), a joint venture between the Helmholtz Centre for Infection Research (HZI) Hannover Medical School (MHH)  Hannover 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