{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:07:38Z","timestamp":1792591658105,"version":"4.5.0"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T00:00:00Z","timestamp":1665187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Treatment of inflammatory skin diseases, including atopic dermatitis (AD) and psoriasis, is undergoing transformative changes, highlighting the need to develop experimental models of skin inflammation in humans to predict treatment responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We topically or intradermally administered four common sensitizers (dust mite (DM), diphencyprone (DPCP), nickel (Ni), and purified protein derivative (PPD)) to the backs of 40 healthy patients and the skin hypersensitivity response was biopsied and evaluated using immunohistochemistry, RNA\u2010seq, and RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All agents induced strong increases in cellular infiltrates (T\u2010cells and dendritic cells) as compared to untreated skin (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), with variable T helper polarization. Overall, DPCP induced the strongest immune responses across all pathways, including innate immunity (IL\u20101\u03b1, IL\u20108), Th1 (IFN\u03b3, CXCL10), Th2 (IL\u20105, CCL11), and Th17 (CAMP\/LL37) products, as well as the highest regulatory tone (FOXP3, IL\u201034, IL\u201037) (FDR\u2009&lt;0.01). Nickel induced Th17 (IL\u201017A), Th1 (CXCL10) and Th2 (IL\u20104R) immune responses to a lesser extent than DPCP (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). PPD induced predominantly Th1 (IFN\u03b3, CXCL10, STAT1) and Th17 inflammation (IL\u201017A) (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). DM induced modulation of\u00a0Th2 (IL\u201013, CCL17, CCL18), Th22 (IL\u201022), and Th17\/Th22 (S100A7\/9\/12) pathways (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Barrier defects that characterize both AD and psoriasis were best modeled by DPCP and Ni, followed by PPD, including downregulation of terminal differentiation (FLG, FLG2, LOR, LCEs), tight junction (CLDN1\/CLDN8), and lipid metabolism (FA2H, FABP7)\u2010related markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data imply that DPCP induced the strongest immune response across all pathways, and barrier defects characteristic of AD and psoriasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15538","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T10:36:23Z","timestamp":1664534183000},"page":"178-191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Delayed type hypersensitivity reactions to various allergens may differently model inflammatory skin 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