{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:24:14Z","timestamp":1789568654147,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T00:00:00Z","timestamp":1684972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011860","name":"Campaign Urging Research for Eosinophilic Disease","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011860","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pericles.pericles-gcp.literatumonline.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eosinophilic esophagitis (EoE) involves a chronic immune\u2010mediated response to dietary antigens. Recent work identifies T\u2010cell clonality in children with EoE, however, it is unknown whether this is true in adults or whether there is a restricted food\u2010specific T\u2010cell repertoire. We sought to confirm T\u2010cell receptor (TCR) clonality in EoE and assess for differences with specific food triggers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bulk TCR sequencing was performed on mRNA isolated from esophageal biopsies obtained from adults and children with EoE (<jats:italic>n<\/jats:italic>\u2009=\u200915) who had food triggers confirmed by endoscopic evaluation. Non\u2010EoE adult and pediatric controls (<jats:italic>n<\/jats:italic>\u2009=\u200910) were included. Differences in TCR clonality by disease and treatment status were assessed. Shared and similar V\u2010J\u2010CDR3s were assessed based on specific food triggers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Active EoE biopsies from children but not adults displayed decreased unique TCR\u03b1\/\u03b2 clonotypes and increased relative abundance of TCRs comprising &gt;1% of the total compared to non\u2010EoE controls and paired inactive EoE samples. Among patients in which baseline, post diet elimination, and food trigger reintroduction samples (<jats:italic>n<\/jats:italic>\u2009=\u20096) were obtained, we observed ~1% of TCRs were shared only between pre\u2010diet elimination and trigger reintroduction. Patients with a shared EoE trigger (milk) had a greater degree of shared and similar TCRs compared to patients with differing triggers (seafood, wheat, egg, soy).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We confirmed relative clonality in children but not adults with active EoE and identified potential food\u2010specific TCRs, particularly for milk\u2010triggered EoE. Further studies are needed to better identify the broad TCR repertoire relevant to food triggers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.15773","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T05:50:12Z","timestamp":1684475412000},"page":"2487-2496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bulk T\u2010cell receptor sequencing confirms clonality in pediatric eosinophilic esophagitis and identifies a food\u2010specific repertoire"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2872-6957","authenticated-orcid":false,"given":"Rethavathi","family":"Janarthanam","sequence":"first","affiliation":[{"name":"Division of Gastroenterology, Hepatology &amp; Nutrition, Ann &amp; Robert H. Lurie Children's Hospital of Chicago Northwestern University Feinberg school of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9570-3806","authenticated-orcid":false,"given":"Fei Li","family":"Kuang","sequence":"additional","affiliation":[{"name":"Division of Allergy\u2010Immunology Northwestern University Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8408-3488","authenticated-orcid":false,"given":"Angelika","family":"Zalewski","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine Northwestern University Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Amsden","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Hepatology &amp; Nutrition, Ann &amp; Robert H. 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