{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T13:20:32Z","timestamp":1793884832948,"version":"4.7.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003554","name":"Lundbeckfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006310","name":"Medicinska Forskningsr\u00e5det","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Respiratory virus infections are main triggers of asthma exacerbations. Tezepelumab, an anti\u2010TSLP mAb, reduces exacerbations in patients with asthma, but the effect of blocking TSLP on host epithelial resistance and tolerance to virus infection is not known.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To examine effects of blocking TSLP in patients with asthma on host resistance (IFN\u03b2, IFN\u03bb, and viral load) and on the airway epithelial inflammatory response to viral challenge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Bronchoalveolar lavage fluid (BALF,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200939) and bronchial epithelial cells (BECs) were obtained from patients with uncontrolled asthma before and after 12\u2009weeks of tezepelumab treatment (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200913) or placebo (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200913). BECs were cultured in\u00a0vitro and exposed to the viral infection mimic poly(I:C) or infected by rhinovirus (RV). Alarmins, T2\u2010 and pro\u2010inflammatory cytokines, IFN\u03b2 IFN\u03bb, and viral load were analyzed by RT\u2010qPCR and multiplex ELISA before and after stimulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IL\u201033 expression in unstimulated BECs and IL\u201033 protein levels in BALF were reduced after 12\u2009weeks of tezepelumab. Further, IL\u201033 gene and protein levels decreased in BECs challenged with poly(I:C) after tezepelumab whereas TSLP gene expression remained unaffected. Poly(I:C)\u2010induced IL\u20104, IL\u201013, and IL\u201017A release from BECs was also reduced with tezepelumab whereas IFN\u03b2 and IFN\u03bb expression and viral load were unchanged.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Blocking TSLP with tezepelumab in\u00a0vivo in asthma reduced the airway epithelial inflammatory response including IL\u201033 and T2 cytokines to viral challenge without affecting anti\u2010viral host resistance. Our results suggest that blocking TSLP stabilizes the bronchial epithelial immune response to respiratory viruses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.15918","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T05:11:04Z","timestamp":1697519464000},"page":"656-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Tezepelumab decreases airway epithelial IL\u201033 and T2\u2010inflammation in response to viral stimulation in patients with asthma"],"prefix":"10.1111","volume":"79","author":[{"given":"A.","family":"Sverrild","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine University Hospital Bispebjerg  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5368-4060","authenticated-orcid":false,"given":"S.","family":"Cerps","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Nieto\u2010Fontarigo","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Lund University  Lund Sweden"},{"name":"BioLympho Research group, Department of Biochemistry and Molecular Biology, Faculty of Biology\u2010Biological Research Centre (CIBUS) Universidade de Santiago de Compostela  Santiago de Compostela Spain"},{"name":"Translational Research in Airway Diseases Group (TRIAD) Health Research Institute of Santiago de Compostela (IDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ramu","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hvidtfeldt","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University Hospital Bispebjerg  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1286-2022","authenticated-orcid":false,"given":"M.","family":"Menzel","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kearley","sequence":"additional","affiliation":[{"name":"Bioscience, Research &amp; Early Development, Respiratory &amp; Immunology BioPharmaceuticals R&amp;D, AstraZeneca  Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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