{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:15:57Z","timestamp":1787066157606,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      15\u2010oxo\u2010eicosatetraenoic acid (15\u2010oxo\u2010ETE), is a product of arachidonic acid (AA) metabolism in the 15\u2010lipoxygenase\u20101 (15\u2010LOX\u20101) pathway. 15\u2010oxo\u2010ETE was overproduced in the nasal polyps of patients with nonsteroidal anti\u2010inflammatory drug\u2013exacerbated respiratory disease (N\u2010ERD). In this study we investigated the systemic biosynthesis of 15\u2010oxo\u2010ETE and leukotriene E\n                      <jats:sub>4<\/jats:sub>\n                      (LTE\n                      <jats:sub>4<\/jats:sub>\n                      ) and assessed their diagnostic value to identify patients with N\u2010ERD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study included 64 patients with N\u2010ERD, 59 asthmatics who tolerated aspirin well (ATA), and 51 healthy controls. A thorough clinical characteristics of asthmatics included computed tomography of paranasal sinuses. Plasma and urinary 15\u2010oxo\u2010ETE levels, and urinary LTE\n                      <jats:sub>4<\/jats:sub>\n                      excretion were measured using high\u2010performance liquid chromatography and tandem mass spectrometry. Repeatability and precision of the measurements were tested.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Plasma 15\u2010oxo\u2010ETE levels were the highest in N\u2010ERD (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001). A receiver operator characteristic (ROC) revealed that 15\u2010oxo\u2010ETE had certain sensitivity (64.06% in plasma, or 88.24% in urine) for N\u2010ERD discrimination, while the specificity was rather limited. Modeling of variables allowed to construct the Aspirin Hypersensitivity Diagnostic Index (AHDI) based on urinary LTE\n                      <jats:sub>4<\/jats:sub>\n                      \u2010to\u201015\u2010oxo\u2010ETE excretion corrected for sex and the Lund\u2010Mackay score of chronic rhinosinusitis. AHDI outperformed single measurements in discrimination of N\u2010ERD among asthmatics with an area under ROC curve of 0.889, sensitivity of 81.97%, specificity of 87.23%, and accuracy of 86.87%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We confirmed 15\u2010oxo\u2010ETE as a second to cysteinyl leukotrienes biomarker of N\u2010ERD. An index based on these eicosanoids corrected for sex and Lund\u2010Mackay score has a similar diagnostic value as gold standard oral aspirin challenge in the studied group of patients with asthma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16281","type":"journal-article","created":{"date-parts":[[2024,8,24]],"date-time":"2024-08-24T01:45:25Z","timestamp":1724463925000},"page":"534-544","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Aspirin hypersensitivity diagnostic index (\n                    <scp>AHDI<\/scp>\n                    ): In\u00a0vitro test for diagnosing of N\u2010\n                    <scp>ERD<\/scp>\n                    based on urinary 15\u2010oxo\u2010\n                    <scp>ETE<\/scp>\n                    and\n                    <scp>\n                      LTE\n                      <sub>4<\/sub>\n                    <\/scp>\n                    excretion"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8994-0036","authenticated-orcid":false,"given":"Lucyna","family":"Mastalerz","sequence":"first","affiliation":[{"name":"2nd Department of Internal Medicine Jagiellonian University Medical College  Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Tr\u0105d","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine Jagiellonian University Medical College  Krakow Poland"},{"name":"Doctoral School of Medical and Health Sciences Jagiellonian University  Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Szatkowski","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine Jagiellonian University Medical College  Krakow 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