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Ideal allergen products for skin prick testing (SPT) and allergen immunotherapy (AIT) should contain the complete set of allergens of the respective allergen sources to cover all sensitization profiles. However, commercially available allergen products were shown to vary regarding their allergen composition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The qualitative allergen composition of different SPT and AIT products produced from pollen of grasses, birch, mugwort and from house dust mites was assessed by a consistent high\u2010resolution liquid chromatography\u2010coupled tandem mass spectrometry method (LC\u2013MS\/MS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All major, mid\u2010tier and most minor allergens were detected in each of the investigated three batches of SPT and AIT products, demonstrating the completeness of the allergen composition and a high degree of batch\u2010to\u2010batch consistency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first study using a single consistent high\u2010resolution LC\u2013MS\/MS method to provide solid data on the qualitative allergen composition of SPT and AIT products manufactured from various common allergen sources. The applied method showed high reliability in qualitative batch\u2010to\u2010batch consistency testing and can be performed fast and with high throughput. High\u2010resolution LC\u2013MS\/MS is applicable for process development and quality control to ensure market availability of allergen products corresponding to the composition of the respective natural allergen sources.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.16339","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T10:50:49Z","timestamp":1729767049000},"page":"3489-3500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Improved quality control of allergen products: Assessing the molecular allergen composition by mass spectrometry"],"prefix":"10.1111","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3927-1645","authenticated-orcid":false,"given":"Steffen","family":"Augustin","sequence":"first","affiliation":[{"name":"Allergopharma GmbH &amp; Co  Reinbek Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Eichhorn","sequence":"additional","affiliation":[{"name":"Merck KGaA  Darmstadt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Wald","sequence":"additional","affiliation":[{"name":"Allergopharma GmbH &amp; Co  Reinbek Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Fischer","sequence":"additional","affiliation":[{"name":"Merck KGaA  Darmstadt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1555-9379","authenticated-orcid":false,"given":"Marek","family":"Jutel","sequence":"additional","affiliation":[{"name":"ALL\u2010MED Medical Research Institute  Wroclaw Poland"},{"name":"Department of Clinical Immunology Wroclaw Medical University  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-9639","authenticated-orcid":false,"given":"Oliver","family":"Pfaar","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Section of Rhinology and Allergy, University Hospital Marburg Philipps\u2010Universit\u00e4t Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Willers","sequence":"additional","affiliation":[{"name":"Allergopharma GmbH &amp; Co  Reinbek Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15023"},{"key":"e_1_2_11_3_1","first-page":"1","article-title":"Allergen immunotherapy: past, present and future","volume":"10","author":"Durham SR","year":"2022","journal-title":"Nat Rev Immunol"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14077"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13317"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.waojou.2019.100080"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000230518"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(81)90199-8"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000231548"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01756.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2015.05.004"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12303"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.10.045"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.03.024"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.07.010"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.651500"},{"key":"e_1_2_11_17_1","volume-title":"2008 European Medicines Agency (EMEA); Committee for Medicinal Products of Human Use (CHMP)","author":"Guideline on Allergen Products: Production and Quality Issues","year":"2008"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aller.2017.09.002"},{"key":"e_1_2_11_19_1","unstructured":"European Pharmacopoeia. 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