{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:01:40Z","timestamp":1781103700469,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,28]],"date-time":"2024-09-28T00:00:00Z","timestamp":1727481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013348","name":"Innosuisse - Schweizerische Agentur f\u00fcr Innovationsf\u00f6rderung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Peanut allergy is among the most severe and common food allergies. The diagnosis has a significant impact on the quality of life for patients and their families. An effective management approach depends on accurate, safe, and easily implementable diagnostic methods. We previously developed a cell\u2010based assay using Hoxb8 mast cells (Hoxb8 MCs) aimed at improving clinical allergy diagnosis. In this study, we assessed its diagnostic performance by measuring blinded sera from a prospectively enrolled and pre\u2010validated peanut allergy cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hoxb8 MCs were passively sensitized with sera from peanut\u2010allergic and peanut tolerant children and adolescents (<jats:italic>n<\/jats:italic>\u2009=\u2009112). Degranulation of Hoxb8 MCs was quantified upon stimulation with dose\u2010titrated peanut extract by means of flow cytometry, using CD107a as activation marker. The results from the Hoxb8 mast cell activation test (Hoxb8 MAT) were compared to established diagnostic assays such as the skin prick test (SPT), specific IgE (sIgE) levels, and the basophil activation test (BAT). Additionally, serum samples from BAT nonresponders were assessed with the Hoxb8 MAT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hoxb8 MAT displayed a robust dose\u2010dependent activation to peanut extract, with a cutoff value of \u22645.2% CD107a positive cells. The diagnostic accuracy was highest at allergen concentrations \u2265100\u2009ng\/mL, with an area under the receiver operating characteristic curve (AUROC) of 0.97, 93% sensitivity, and 96% specificity, outperforming traditional SPT and sIgE tests. When compared to BAT, Hoxb8 MAT exhibited comparable diagnostic efficacy. Moreover, sera from BAT nonresponders were accurately classified into allergics and nonallergics by the Hoxb8 MAT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The Hoxb8 MAT demonstrated a very good diagnostic precision in patients prospectively assessed for peanut allergy comparable to the fresh whole blood\u2010based BAT. Additionally, it demonstrated its value for accurate classification of BAT nonresponders into allergic and nonallergic individuals. Further investigations into its utility in the routine clinical setting are warranted.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.16341","type":"journal-article","created":{"date-parts":[[2024,9,28]],"date-time":"2024-09-28T09:04:23Z","timestamp":1727514263000},"page":"215-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinical utility analysis of the Hoxb8 mast cell activation test for the diagnosis of peanut allergy"],"prefix":"10.1111","volume":"80","author":[{"given":"Noemi","family":"Bachmeier\u2010Zb\u00e4ren","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology University Hospital Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alper","family":"Celik","sequence":"additional","affiliation":[{"name":"Centre for Computational Medicine Hospital for Sick Children  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"van Brummelen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology University Hospital Bern  Bern Switzerland"},{"name":"Department for BioMedical Research, Lung Precision Medicine (LPM) University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Roos","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology University Hospital Bern  Bern Switzerland"},{"name":"Department for BioMedical Research, Lung Precision Medicine (LPM) University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Steinmann","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology University Hospital Bern  Bern Switzerland"},{"name":"Department for BioMedical Research, Lung Precision Medicine (LPM) University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9264-1881","authenticated-orcid":false,"given":"Jennifer A.","family":"Hoang","sequence":"additional","affiliation":[{"name":"Translational Medicine, Research Institute Hospital for Sick Children  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Yin","sequence":"additional","affiliation":[{"name":"Translational Medicine, Research Institute Hospital for Sick Children  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Ditlof","sequence":"additional","affiliation":[{"name":"Department of Immunology, Temerty Faculty of Medicine University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Duan","sequence":"additional","affiliation":[{"name":"Translational Medicine, Research Institute Hospital for Sick Children  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5320-4232","authenticated-orcid":false,"given":"Julia E. 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