{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:27:01Z","timestamp":1791386821770,"version":"4.3.3"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009469","name":"Conseil R\u00e9gional du Centre-Val de Loire","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009469","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Severe refractory, neutrophilic asthma remains an unsolved clinical problem. STING agonists induce a neutrophilic response in the airways, suggesting that STING activation may contribute to the triggering of neutrophilic exacerbations. We aim to determine whether STING\u2010induced neutrophilic lung inflammation mimics severe asthma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed new models of neutrophilic lung inflammation induced by house dust mite (HDM) plus STING agonists diamidobenzimidazole (diABZI) or cGAMP in wild\u2010type, and conditional\u2010STING\u2010deficient mice. We measured DNA damage, cell death, NETs, cGAS\/STING pathway activation by immunoblots, N1\/N2 balance by flow cytometry, lung function by plethysmography, and Th1\/Th2 cytokines by multiplex. We evaluated diABZI effects on human airway epithelial cells from healthy or patients with asthma, and validated the results by transcriptomic analyses of rhinovirus infected healthy controls vs patients with asthma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DiABZI administration during HDM challenge increased airway hyperresponsiveness, neutrophil recruitment with prominent NOS2\n                      <jats:sup>+<\/jats:sup>\n                      ARG1\n                      <jats:sup>\u2212<\/jats:sup>\n                      type 1 neutrophils, protein extravasation, cell death by PANoptosis, NETs formation, extracellular dsDNA release, DNA sensors activation, IFN\u03b3, IL\u20106 and CXCL10 release. Functionally, STING agonists exacerbated airway hyperresponsiveness. DiABZI caused DNA and epithelial barrier damage, STING pathway activation in human airway epithelial cells exposed to HDM, in line with DNA\u2010sensing and PANoptosis pathways upregulation and tight\u2010junction downregulation induced by rhinovirus challenge in patients with asthma.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study identifies that triggering STING in the context of asthma induces cell death by PANoptosis, fueling the flame of inflammation through a mixed Th1\/Th2 immune response recapitulating the features of severe asthma with a prognostic signature of type 1 neutrophils.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16369","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T12:24:36Z","timestamp":1730118276000},"page":"715-737","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<scp>STING<\/scp>\n                    \u2010dependent induction of neutrophilic asthma exacerbation in response to house dust mite"],"prefix":"10.1111","volume":"80","author":[{"given":"Yasmine","family":"Messaoud\u2010Nacer","sequence":"first","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Culerier","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Rose","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Maillet","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Boussad","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Veront","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Savigny","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-9342","authenticated-orcid":false,"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[{"name":"Centre d'Immunoph\u00e9nomique (CIPHE) Aix Marseille Universit\u00e9, INSERM, CNRS  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7341-9764","authenticated-orcid":false,"given":"Urszula","family":"Radzikowska","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich  Davos Switzerland"},{"name":"Christine K\u00fchne\u2014Center for Allergy Research and Education (CK\u2010CARE) Herman\u2010 Burchard\u2010Strasse 1  Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9710-6685","authenticated-orcid":false,"given":"Milena","family":"Sokolowska","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich  Davos Switzerland"},{"name":"Christine K\u00fchne\u2014Center for Allergy Research and Education (CK\u2010CARE) Herman\u2010 Burchard\u2010Strasse 1  Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel V. L.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Ribeir\u00e3o Preto Medical School University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Edwards","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute Imperial College Londont  London UK"},{"name":"Asthma UK Centre in Allergic Mechanism of Asthma  London UK"},{"name":"Imperial College Healthcare NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2299-868X","authenticated-orcid":false,"given":"David J.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Guy\u2019s Severe Asthma Centre, Guy\u2019s &amp; St Thomas\u2019 NHS Trust  London UK"},{"name":"School of Immunology &amp; Microbial Sciences, King\u2019s College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3009-9200","authenticated-orcid":false,"given":"Sebastian L.","family":"Johnston","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute Imperial College Londont  London UK"},{"name":"Asthma UK Centre in Allergic Mechanism of Asthma  London UK"},{"name":"Imperial College Healthcare NHS Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2897-8230","authenticated-orcid":false,"given":"Bernhard","family":"Ryffel","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2907-7995","authenticated-orcid":false,"given":"Valerie F.","family":"Quesniaux","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6658-414X","authenticated-orcid":false,"given":"Dieudonn\u00e9e","family":"Togbe","sequence":"additional","affiliation":[{"name":"Experimental and Molecular Immunology and Neurogenetics INEM UMR7355 University of Orleans and CNRS  Orleans France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.02.016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2019-0170OC"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2019.101301"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14128"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3786"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.03.018"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2403"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00524-z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900869"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02212"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80911"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04664-5"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215006109"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1244040"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abn6612"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0705-y"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12506"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.047"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42675"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00311"},{"issue":"12","key":"e_1_2_11_22_1","first-page":"1286","article-title":"Neutrophil extracellular traps: mechanisms of formation and role in health and disease","volume":"79","author":"Vorobjeva NV","year":"2014","journal-title":"Biochemistry"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.1943605"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0302-0"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.07.003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.143509"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00538-7"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13619"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.05.038"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4332"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118286"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201406-1039OC"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37470-4"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.10.024"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.08.017"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0151-1"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07953-9"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.00143.x"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2013.5.6.343"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00483-2022"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40820-x"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2019.03.003"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00532"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91947-0"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-022516-034339"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-018-0809-9"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200709-1363OC"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00057710"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3117"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-021-01709-9"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.16369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/all.16369","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/all.16369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T14:50:36Z","timestamp":1771167036000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/all.16369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,28]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/all.16369"],"URL":"https:\/\/doi.org\/10.1111\/all.16369","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,28]]},"assertion":[{"value":"2023-10-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}