{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T18:47:11Z","timestamp":1785869231139,"version":"3.56.0"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic rhinosinusitis with nasal polyps (CRSwNP) is an upper respiratory disease characterized by persistent inflammation of the nasal mucosa. However, the mechanism of abnormal Mucin5AC expression by CRSwNP epithelial cells is not fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We investigated the potential role of yes\u2010associated protein (YAP) underlying the mechanism of excessive epithelial Mucin5AC expression in CRSwNP in a hypoxic model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissue biopsies of CRSwNP (<jats:italic>n<\/jats:italic>\u2009=\u200960), chronic rhinosinusitis without nasal polyps (CRSsNP) (<jats:italic>n<\/jats:italic>\u2009=\u20099) and healthy controls (<jats:italic>n<\/jats:italic>\u2009=\u200930) were investigated together with a well\u2010established hypoxic model of primary human nasal epithelial cells (hNECs). The expression levels of hypoxia inducible factor (HIF)\u20101\u03b1 and YAP, and the effect of the signaling axis on mucus secretion in hNECs were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed a significant elevated expression levels of YAP in patients with CRSwNP and CRSsNP compared to controls. In addition, HIF\u20101\u03b1 expression of CRSwNP was higher than that of control group. Under hypoxic conditions, HIF\u20101\u03b1 was found to regulate the upregulation of YAP in hNECs. Further investigations revealed that HIF\u20101\u03b1 facilitated the activation and nuclear localization of active\u2010YAP by reducing the phosphorylation of YAP. This mechanism appeared to be linked to HIF\u20101\u03b1\u2010mediated inhibition of LATS 1 phosphorylation and subsequent YAP degradation. HIF\u20101\u03b1 was shown to promote the expression of P63 and the levels of Mucin5AC in hNECs by enhancing YAP activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicated that hypoxia enhances YAP activation by decreasing p\u2010LATS 1 and YAP phosphorylation. This has the potential to impact on the proliferation of basal cells and the differentiation of goblet cells in CRSwNP, ultimately leading to a pathological condition characterized by excessive Mucin5AC expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/all.16394","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T14:18:57Z","timestamp":1731507537000},"page":"1271-1285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Hypoxia\u2010reduced\u00a0<scp>YAP<\/scp> phosphorylation enhances expression of <scp>Mucin5AC<\/scp> in nasal epithelial cells of chronic rhinosinusitis with nasal polyps"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6654-4106","authenticated-orcid":false,"given":"Bing","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery, West China Hospital Sichuan University  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