{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T03:49:16Z","timestamp":1782013756441,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004836","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021201","name":"European Academy of Dermatology and Venereology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021201","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002301","name":"Eesti Teadusagentuur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002301","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Dysfunction of the skin barrier is regarded as a key event in the initiation and progression of inflammatory skin diseases. In many cases of allergic contact dermatitis (ACD), epidermal\u2010resident memory CD8\n                      <jats:sup>+<\/jats:sup>\n                      T (T\n                      <jats:sub>RM<\/jats:sub>\n                      ) cells play a central role in the immune response to contact allergens. However, if and how allergen\u2010specific CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\n                      <jats:sub>RM<\/jats:sub>\n                      cells affect the expression of skin barrier molecules is not known.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The expression level of skin barrier molecules was determined by RT\u2010qPCR and immunofluorescence in a mouse model of ACD. The role of CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells on the expression of skin barrier molecules was investigated by depletion of CD8\n                      <jats:sup>+<\/jats:sup>\n                      cells. Human primary keratinocytes were used to assess the direct effect of IFN\u2010\u03b3 and contact allergen on their expression of skin barrier molecules.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sensitization with the contact allergen 1\u2010fluoro\u20102,4\u2010dinitrobenzene (DNFB) resulted in epidermal accumulation of CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\n                      <jats:sub>RM<\/jats:sub>\n                      cells and prolonged upregulation of\n                      <jats:italic>Ifng<\/jats:italic>\n                      and downregulation of keratin 5 (\n                      <jats:italic>Krt5<\/jats:italic>\n                      ) and\n                      <jats:italic>Krt14<\/jats:italic>\n                      even after complete macroscopic remission of the inflammatory response. Challenge with DNFB lead to an additionally rapid downregulation of\n                      <jats:italic>Krt5<\/jats:italic>\n                      and\n                      <jats:italic>Krt14<\/jats:italic>\n                      and the downregulation of several other skin barrier molecules. Depletion of CD8\n                      <jats:sup>+<\/jats:sup>\n                      cells abolished both the prolonged and rapid downregulation of skin barrier molecules. In keratinocytes, IFN\u2010\u03b3 and contact allergen synergistically down\u2010regulated the expression of KRT5 and KRT14.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\n                      <jats:sub>RM<\/jats:sub>\n                      cells contribute to a prolonged reduction in the expression of skin barrier molecules, which might exacerbate allergen permeation and the inflammatory response during succeeding exposures of the skin to allergens and antigens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16421","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T05:31:22Z","timestamp":1734154282000},"page":"1956-1966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["T Cells Induce Prolonged Downregulation of Barrier Molecules in a Mouse Model of Allergic Contact Dermatitis"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8845-0326","authenticated-orcid":false,"given":"Helen","family":"Vaher","sequence":"first","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5757-9365","authenticated-orcid":false,"given":"Anne\u2010Sofie \u00d8.","family":"Gadsb\u00f8ll","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5878-0971","authenticated-orcid":false,"given":"Alexandra T.","family":"Seibel","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3496-9333","authenticated-orcid":false,"given":"Martin","family":"Kongsbak\u2010Wismann","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1595-9698","authenticated-orcid":false,"given":"Rebecca K. D.","family":"Lohmann","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8841-8131","authenticated-orcid":false,"given":"Veronika","family":"Mraz","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7576-3884","authenticated-orcid":false,"given":"Anders B.","family":"Funch","sequence":"additional","affiliation":[{"name":"The LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences 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