{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:02:40Z","timestamp":1791820960402,"version":"4.3.4"},"reference-count":71,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K08550"],"award-info":[{"award-number":["JP22K08550"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>IgE\u2010mediated food allergy is accompanied by mucosal mast cell (MMC) hyperplasia in the intestinal mucosa. Intestinal MMC numbers correlate with the severity of food allergy symptoms. However, the mechanisms by which MMCs proliferate excessively are poorly understood. Here, we clarify the role of newly identified MHC class II (MHCII)\u2010expressing MMCs in the effector phase of IgE\u2010mediated food allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mice reconstituted with MHCII\u2010deficient or wild\u2010type MMCs were used to generate a mouse mode of IgE\u2010mediated food allergy. We assessed the extent of intestinal MMC hyperplasia and the severity of hypothermia in these mice. In addition, we performed in\u00a0vitro antigen presentation assay using induced MHCII\u2010expressing MMCs generated from bone marrow cells to evaluate the effect of CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cell activation on MMC proliferation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In food\u2010allergic mice, we identified the appearance of MHCII\u2010expressing MMCs in the intestinal mucosa and showed that MMC hyperplasia was suppressed in mice with MHCII\u2010deficient MMCs compared to mice with wild\u2010type MMCs. In\u00a0vitro assays demonstrated that MHCII\u2010expressing MMCs incorporate food antigens directly and through the high\u2010affinity IgE receptor Fc\u03b5RI\u2010mediated endocytosis and activate antigen\u2010specific CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells from food\u2010allergic mice by antigen presentation. Activated CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells secrete IL\u20104 and large amounts of IL\u20105, which enhance production of the mast cell growth factor IL\u20109 by IL\u201033\u2010activated MMCs. Excess IL\u20109 causes excessive MMC proliferation, leading to the development of MMC hyperplasia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Antigen presentation to CD4\n                      <jats:sup>+<\/jats:sup>\n                      T cells by MHCII\u2010expressing MMCs triggers intestinal MMC hyperplasia and exacerbates IgE\u2010mediated food allergy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/all.16477","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T09:01:24Z","timestamp":1737968484000},"page":"2297-2309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>MHC<\/scp>\n                    Class\n                    <scp>II<\/scp>\n                    \u2010Expressing Mucosal Mast Cells Promote Intestinal Mast Cell Hyperplasia in a Mouse Model of Food Allergy"],"prefix":"10.1111","volume":"80","author":[{"given":"Kenji","family":"Oishi","sequence":"first","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5317-5950","authenticated-orcid":false,"given":"Nobuhiro","family":"Nakano","sequence":"additional","affiliation":[{"name":"Atopy (Allergy) Research Center Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masamu","family":"Ota","sequence":"additional","affiliation":[{"name":"Atopy (Allergy) Research Center Juntendo University Graduate School of Medicine  Tokyo Japan"},{"name":"Department of Biological Science and Technology, Faculty of Advanced Engineering Tokyo University of Science  Tokyo 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